SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery
SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery
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DOI：
10.1093/pcp/pcv076
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发表时间：
2016-02-01
影响因子：
4.9
通讯作者：
Ueda, Takashi
中科院分区：
文献类型：
--
作者：
Kanazawa, Takehiko;Era, Atsuko;Ueda, Takashi
膜运输途径已经以特定的方式为每个真核细胞谱系多样化，可能是为了在生物体中实现特定的功能。在绿色植物中，比较基因组学已经支持了这样的可能性，即terrestrialization和/或多细胞化可能与膜运输途径的精细化和多样化相关，这已经通过膜运输的机械组件所需的基因数量的扩展来实现，包括可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）蛋白。然而，关于膜运输途径在基底陆地植物谱系的信息仍然有限。在本研究中，我们进行了广泛的分析陷阱分子，介导靶膜和运输囊泡或供体细胞器之间的膜融合，地钱。分枝多形藻基因组含有36个SNARE蛋白的至少34个基因，包括陆地植物谱系中共享的具有低冗余度的SNARE蛋白的基本组。我们通过在M.结果表明，在拟南芥中，一些SNARE蛋白被靶向到与其直链产物不同的隔室。例如，MpSYP 12 B定位于油体的表面，这是地钱中独特的细胞器。此外，我们确定了三个VAMP 72成员具有独特的结构特征，其N-末端延伸包含N-豆蔻酰化的共识序列。这些结果表明M.多形藻在进化过程中获得了与新获得的机械组分相关的独特的膜运输途径。
The membrane trafficking pathway has been diversified in a specific way for each eukaryotic lineage, probably to fulfill specific functions in the organisms. In green plants, comparative genomics has supported the possibility that terrestrialization and/or multicellularization could be associated with the elaboration and diversification of membrane trafficking pathways, which have been accomplished by an expansion of the numbers of genes required for machinery components of membrane trafficking, including soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. However, information regarding membrane trafficking pathways in basal land plant lineages remains limited. In the present study, we conducted extensive analyses of SNARE molecules, which mediate membrane fusion between target membranes and transport vesicles or donor organelles, in the liverwort, Marchantia polymorpha. The M. polymorpha genome contained at least 34 genes for 36 SNARE proteins, comprising fundamental sets of SNARE proteins that are shared among land plant lineages with low degrees of redundancy. We examined the subcellular distribution of a major portion of these SNARE proteins by expressing Citrine-tagged SNARE proteins in M. polymorpha, and the results showed that some of the SNARE proteins were targeted to different compartments from their orthologous products in Arabidopsis thaliana. For example, MpSYP12B was localized to the surface of the oil body, which is a unique organelle in liverworts. Furthermore, we identified three VAMP72 members with distinctive structural characteristics, whose N-terminal extensions contain consensus sequences for N-myristoylation. These results suggest that M. polymorpha has acquired unique membrane trafficking pathways associated with newly acquired machinery components during evolution.