MOUSE MYELOMA PROTEINS WITH ANTIHAPTEN ANTIBODY ACTIVITY . PROTEIN PRODUCED BY PLASMA CELL TUMOR MOPC-315
MOUSE MYELOMA PROTEINS WITH ANTIHAPTEN ANTIBODY ACTIVITY . PROTEIN PRODUCED BY PLASMA CELL TUMOR MOPC-315
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DOI：
10.1021/bi00851a048
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发表时间：
1968-01-01
期刊：
影响因子：
2.9
通讯作者：
POTTER, M
中科院分区：
文献类型：
--
作者：
EISEN, HN;SIMMS, ES;POTTER, M
材料与方法瘤的诱导及瘤蛋白的纯化。肿瘤MOPC-315在杂合子（7回交代）BALB/c-2小鼠（Potter and Lieberman, 1967a, b）中通过三次腹腔注射0.5 ml Bayol F (Potter and Boyce, 1962)，分别在2、4和6月龄时诱导。在BALB/c-2中，来自C57BL/6菌株的免疫球蛋白重链连锁基团逐渐回交到BALB/c上。在这样的动物中产生的浆细胞肿瘤，只表达来自两条重链连锁组染色体中的一条的免疫球蛋白重链基因（Warner et al., 1966）。MOPC-315产生的IgA免疫球蛋白携带BALB/c重链连锁基团的A12决定因子（Lieberman and Potter, 1966）。MOPC-315不生产1术语：免疫球蛋白的命名和缩写符合世界卫生组织推荐的名称和缩写。世界卫生组织，30,447(1964))。浆细胞肿瘤被称为MOPC-315，它产生的免疫球蛋白被称为315蛋白，或简称315。
Materials and MethodsInduction of the Tumor and Purification of the My-eloma Protein. Tumor MOPC-315 was induced in a heterozygous (seven backcross generations) BALB/c-2 mouse (Potter and Lieberman, 1967a, b) by three intraperito-neal injections of 0.5 ml of Bayol F (Potter and Boyce, 1962), given at 2, 4, and 6 months of age. In BALB/c-2 the immunoglobulin heavy-chain linkage group from the C57BL/6 strain is introgressively backcrossed onto BALB/c. A plasma cell tumor arising in such an animal expresses an immunoglobulin heavy-chain gene from only one of the two chromosomes with the heavy-chain linkage group (Warner et al., 1966). The IgA immunoglobulin made by MOPC-315 carries the A12 deter-minant (Lieberman and Potter, 1966) of the heavy-chain linkage group of BALB/c. MOPC-315 does not produce1 Terminology: Nomenclature and abbreviations for immunoglobulins correspond to those recommended by the World Health Organization (Bull. World Health Organ. 30, 447 (1964)). The plasma cell tumor is referred to as MOPC-315, and the immunoglobulin it produces as protein 315, or simply 315.