DUN1 ENCODES A PROTEIN-KINASE THAT CONTROLS THE DNA-DAMAGE RESPONSE IN YEAST
DUN1 ENCODES A PROTEIN-KINASE THAT CONTROLS THE DNA-DAMAGE RESPONSE IN YEAST
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DOI：
10.1016/0092-8674(93)90321-g
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发表时间：
1993-12-17
期刊：
影响因子：
64.5
通讯作者：
ELLEDGE, SJ
中科院分区：
文献类型：
--
作者：
ZHOU, Z;ELLEDGE, SJ
DNA损伤诱导了许多增强DNA修复能力的基因的表达。我们研究了DNA损伤诱导核糖核苷酸还原酶亚基RNR3转录的机制。鉴定了5个dna损伤不诱导突变体的互补组。它们都对DNA损伤很敏感。dun1突变体对RNR1和RNR2的诱导也有缺陷，但对其他基因的诱导却很熟练，这表明存在至少两种不同的DNA损伤诱导途径。DUN1编码一种核蛋白激酶，它也是一种磷蛋白。Dun1的磷酸化以Dun1依赖的方式响应DNA损伤而增加，这表明自磷酸化活性增加。这些结果证实了真核生物存在由蛋白磷酸化调控的SOS反应。
DNA damage induces the expression of many genes proposed to enhance DNA repair capacities. We investigated the mechanism by which DNA damage induces transcription of RNR3, a subunit of ribonucleotide reductase. Five complementation groups of DNA-damage uninducible (dun) mutants were identified. Each is sensitive to DNA damage. dun1 mutants are also defective for RNR1 and RNR2 induction but are proficient for induction of other genes, defining the existence of at least two distinct DNA damage induction pathways. DUN1 encodes a nuclear protein kinase that is also a phosphoprotein. Phosphorylation of Dun1 increases in response to DNA damage in a Dun1-dependent manner, suggesting an increase in autophosphorylation activity. These results establish the existence of a eukaryotic SOS response regulated by protein phosphorylation.