Regulation of the postburn wound inflammatory response by γδ T-cells
Regulation of the postburn wound inflammatory response by γδ T-cells
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DOI：
10.1097/shk.0b013e318034264c
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发表时间：
2007-09-01
期刊：
影响因子：
3.1
通讯作者：
Schwacha, Martin G.
中科院分区：
文献类型：
--
作者：
Daniel, TanJanika;Thobe, Ljoern M.;Schwacha, Martin G.
烧伤部位的愈合是患者从这种形式的创伤中成功恢复的关键组成部分。我们实验室以前的研究表明，γ δ T细胞通过产生生长因子在烧伤伤口愈合中很重要。然而，这些细胞在烧伤创面炎症中的作用仍然未知。为了研究这一点，使野生型（WT）和γ δ T细胞受体缺陷型（6 TCR-/-）C57 BL/6雄性小鼠经受烧伤或假手术。通过将聚乙烯醇海绵植入受伤小鼠的烧伤部位下方或假手术小鼠的未受伤皮肤下方来收集伤口细胞。在损伤后3天，收集浸润细胞、伤口液和皮肤用于分析。烧伤显著增加皮肤肿瘤坏死因子-α（TNF-α）和单核细胞趋化蛋白1水平。在WT小鼠中，非烧伤伤口和烧伤伤口之间的浸润细胞数量相似。相比之下，6只TCR-/-小鼠显示细胞浸润减少6倍。与非烧伤创面相比，WT小鼠烧伤创面TNF-α、单核细胞趋化蛋白1和白细胞介素6含量显著增加，而在6只TCR-/-小鼠中，未观察到烧伤诱导的TNF-α和白细胞介素6含量增加。在WT小鼠中，在损伤后3天的伤口细胞浸润缺乏γ δ T细胞。它主要是髓系起源，表达高水平的CD 11b和F4/80。总之，这些研究结果表明，常驻γ δ T细胞在热损伤后伤口部位炎症细胞的募集和炎症反应的调节中是重要的。
Healing of the burn injury site is a critical component of the patient's successful recovery from this form of trauma. Previous studies from our laboratory have demonstrated that gamma delta T-cells via the production of growth factors are important in burn wound healing. Nonetheless, the role of these cells in burn wound inflammation remains unknown. To study this, wild-type (WT) and gamma delta T-cell receptor deficient (6 TCR-/-) C57BL/6 male mice were subjected to burn injury or sham procedure. Wound cells were collected by implantation of polyvinyl alcohol sponges beneath the burn site in injured mice or beneath uninjured skin in sham mice. At 3 days after injury, infiltrating cells, wound fluid, and skin were collected for analysis. Burn injury markedly increased skin tumor necrosis factor-alpha (TNF-alpha) and monocyte chemoattractant protein 1 levels. In WT mice, the numbers of infiltrating cells were similar between nonburn wounds and burn wounds. In contrast, 6 TCR-/- mice displayed a 6-fold reduction in the cellular infiltrate. Burn injury in WT mice caused a marked increase in burn wound TNF-a, monocyte chemoattractant protein 1, and interleukin 6 content as compared with nonburn wounds, whereas in 6 TCR-/- mice, the burn-induced increase of TNF-a and interleukin 6 was not observed. The wound cell infiltrate at 3 days postinjury was devoid of gamma delta T-cells in WT mice. It was predominately of myeloid origin expressing high levels of CD11b and F4/80. In conclusion, these findings suggest that resident gamma delta T-cells are important in the recruitment of inflammatory cells and regulation of the inflammatory response at the wound site after thermal injury.