The relationship of nicotinamide adenine dinucleotide to the chondrogenic differentiation of limb mesenchymal cells.
The relationship of nicotinamide adenine dinucleotide to the chondrogenic differentiation of limb mesenchymal cells.
复制标题
烟酰胺腺嘌呤二核苷酸与肢体间充质细胞软骨分化的关系。
DOI：
10.1016/0012-1606(85)90448-8
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发表时间：
1985
影响因子：
2.7
通讯作者：
Caplan,AI
中科院分区：
文献类型：
--
作者：
Midura,RJ;Cherney,BW;Caplan,AI
鸡胚肢体间充质细胞的烟酰胺腺嘌呤二核苷酸（NAD）含量被推测通过调节adp核糖基化来控制这些细胞的分化。为了验证这一假设，我们监测了[35S]硫酸盐掺入蛋白多糖的情况，以此来评估培养的肢体间充质细胞在烟酰胺和烟酸处理下的成软骨表达，以提高细胞NAD水平，或用烟酰胺和苯酰胺化合物抑制adp核糖基化。本研究的结果表明，血清成分调节这些化学制剂与肢体间充质细胞之间的相互作用，因此，使在血清存在下进行的实验的解释复杂化。用化学定义的培养基促进肢体间充质细胞的体外增殖和分化，结果表明：(1)细胞NAD含量与培养的肢体间充质细胞的成软骨表达之间不存在明确的相关性；(2)烟酰胺和苯酰胺化合物降低细胞增殖，并且在高剂量的实验中，显著降低肢体间充质细胞培养的成软骨；(3)肢体间充质细胞在培养初期对苯甲酰胺化合物的敏感性增强，这与细胞adp -核糖基化活性的短暂增加和初始软骨分化的时间一致。这些结果表明，NAD不控制肢体间充质细胞的分化，而adp核糖基化是肢体间充质细胞分化的一个完整组成部分，尽管不是控制组成部分。提出了adp核糖基化在肢体间充质细胞分化过程中的作用的模型。
The nicotinamide adenine dinucleotide (NAD) content of mesenchymal cells from the embryonic chick limb has been hypothesized to control the differentiation of these cells by modulation of ADP-ribosylations. To test this hypothesis, [35S]sulfate incorporation into proteoglycans was monitored as an estimate of the chondrogenic expression of cultured limb mesenchymal cells treated with nicotinamide and nicotinic acid to elevate cellular NAD levels or with nicotinamide and benzamide compounds to inhibit ADP-ribosylations. The results of this study indicated that serum component(s) modulate the interactions between these chemical agents and limb mesenchymal cells and, thus, complicate the interpretations of experiments performed in the presence of serum. With a chemically defined medium that promotes limb mesenchymal cell proliferation and differentiationin vitro, it was demonstrated that: (1) no clear correlation exists between cellular NAD content and the chondrogenic expression of cultured limb mesenchymal cells, (2) nicotinamide and benzamide compounds reduce cell proliferation and, at the higher doses tested, considerably reduce chondrogenesis in limb mesenchymal cell cultures, and (3) limb mesenchymal cells exhibit an enhanced susceptibility to benzamide compounds at a time very early in the culture period which temporally coincides with a transient increase in cellular ADP-ribosylation activity and initial chondrogenic differentiation. These results suggest that NAD does not control the differentiation of limb mesenchymal cells and that ADP-ribosylations are an integral, though not controlling, component of limb mesenchyme cytodifferentiation. A model is presented which proposes a role for ADP-ribosylations during the differentiation of limb mesenchymal cells.