Ascorbic acid supplementation improved antibody response to infectious bursal disease vaccination in chickens
Ascorbic acid supplementation improved antibody response to infectious bursal disease vaccination in chickens
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DOI：
10.1093/ps/79.5.680
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发表时间：
2000-05-01
期刊：
影响因子：
4.4
通讯作者：
Wu, CC
中科院分区：
文献类型：
--
作者：
Amakye-Anim, J;Lin, TL;Wu, CC
本研究的目的是确定在饮食中添加抗坏血酸（AA）是否会对鸡的传染性法氏囊病（IBD）疫苗接种以预防传染性法氏囊病病毒（IBDV）感染产生有益影响。 240只无特定病原体（SPF）鸡被分为八个实验组。使用完全随机设计中的 2 x 2 x 2 因子排列；饮食中添加 1,000 ppm 的 AA、疫苗接种和攻击是主要效果。攻击前和攻击后10天，测定血清AA浓度、血清皮质酮浓度、针对IBDV的ELISA抗体滴度、体重、法氏囊与体重(B:B)比率和法氏囊组织学评分(BHS)。饲喂补充 AA 的饮食的未接种疫苗的鸡在攻击后没有表现出临床症状或死亡率，而未补充 AA 的对应鸡的累积发病率为 100%，累积死亡率为 30%。补充AA和疫苗接种的鸡血清AA水平显着高于未补充AA和疫苗接种的鸡（P < 0.05）。疫苗接种后 14 天，与未补充 AA 的鸡相比，补充 AA 的疫苗接种鸡对 IBDV 的 ELISA 滴度显着提高 (P < 0.05)。与未补充 AA 的鸡相比，补充抗坏血酸的鸡，尤其是那些也接种疫苗的鸡，体重增加得更高。补充抗坏血酸的鸡受到IBDV攻击后没有表现出任何临床症状或死亡。结果表明，在饮食中补充 1,000 ppm 的 AA 对 IBD 疫苗接种的抗体反应和体重增加具有有益的影响。
The purpose of the present study was to determine if supplementation of ascorbic acid (AA) to the diet would have a beneficial effect on infectious bursal disease (IBD) vaccination of chickens for protection against infectious bursal disease virus (IBDV) infection. Two hundred forty specific pathogen-free (SPF) chickens were divided into eight experimental groups. A 2 x 2 x 2 factorial arrangement in a completely randomized design was used; AA supplementation at 1,000 ppm in the diet, vaccination, and challenge were the main effects. Prior to challenge and 10 d after challenge, serum AA concentration, serum corticosterone concentration, ELISA antibody titer to IBDV, body weight, bursa-to-body weight (B:B) ratio, and bursal histological score (BHS) were determined. Nonvaccinated chickens fed a diet supplemented with AA did not exhibit clinical signs or mortality following challenge, whereas AA-unsupplemented counterparts had 100% cumulative morbidity and 30%, cumulative mortality. Serum AA levels of AA-supplemented and vaccinated chickens were significantly (P < 0.05) higher than AA-unsupplemented and vaccinated chickens. Fourteen days following vaccination, significantly (P < 0.05) higher ELISA titers to IBDV were observed in vaccinated chickens supplemented with AA as compared to AA-unsupplemented counterparts. Ascorbic acid-supplemented chickens, especially those also vaccinated, had higher body weight gains as compared to the AA-unsupplemented chickens. Ascorbic acid-supplemented chickens challenged with IBDV did not show any clinical signs or mortality. The results suggest that supplementation of AA at 1,000 ppm in the diet has beneficial Effects on antibody response to IBD vaccination and body weight gain.