Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice
Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice
复制标题
组合益生菌促进白介素 10 基因缺陷小鼠的肠上皮屏障功能
DOI：
10.3748/wjg.v20.i16.4636
复制
发表时间：
2014-04-28
影响因子：
4.3
通讯作者：
Qin, Huan-Long
中科院分区：
文献类型：
--
作者：
Shi, Chen-Zhang;Chen, Hong-Qi;Qin, Huan-Long
目的：探讨益生菌联合应用对IL-10基因缺陷小鼠(IL-10 KO)和Caco-2细胞单层的保护作用。IL-10KO和对照组小鼠接受约1.5×10(8)CFU/d的培菲科，连续4wk。然后取结肠，用Ussing Chamber分析上皮屏障功能，同时用ELISA法评估促炎细胞因子。以结肠上皮细胞株Caco-2为研究对象，在体外实验中验证了百菲可的作用。将肠道侵袭性大肠埃希菌(EIEC)和益生菌混合物Bevero或单株益生菌应用于培养的Caco-2单层细胞。通过测量跨上皮电阻值和紧密连接蛋白的表达来确定屏障功能。结果：用倍他可部分恢复IL-10KO小鼠的体重、结肠长度和上皮屏障完整性，使其恢复到野生型水平。此外，接受培菲科治疗的IL-10KO小鼠减少了粘膜中肿瘤坏死因子-α和干扰素-γ的分泌，并减轻了结肠疾病。结论：倍他可减轻IL-10 KO小鼠结肠炎症反应，促进和改善上皮屏障功能，增强对EIEC侵袭的抵抗力，减少促炎细胞因子的分泌。(C)2014年白石登出版集团有限公司。版权所有。
AIM: To investigate the protective effects of combinations of probiotic (Bifico) on interleukin (IL)-10 -gene-deficient (IL-10 KO) mice and Caco-2 cell monolayers.METHODS: IL-10 KO mice were used to assess the benefits of Bifico in vivo. IL-10 KO and control mice received approximately 1.5 x 10(8) cfu/d of Bifico for 4 wk. Colons were then removed and analyzed for epithelial barrier function by Ussing Chamber, while an ELISA was used to evaluate proinflammatory cytokines. The colon epithelial cell line, Caco-2, was used to test the benefit of Bifico in vitro. Enteroinvasive Escherichia coli (EIEC) and the probiotic mixture Bifico, or single probiotic strains, were applied to cultured Caco-2 mono-layers. Barrier function was determined by measuring transepithelial electrical resistance and tight junction protein expression.RESULTS: Treatment of IL-10 KO mice with Bifico partially restored body weight, colon length, and epithelial barrier integrity to wild-type levels. In addition, IL-10 KO mice receiving Bifico treatment had reduced mucosal secretion of tumor necrosis factor-alpha and interferon-gamma, and attenuated colonic disease. Moreover, treatment of Caco-2 monolayers with Bifico or single-strain probiotics in vitro inhibited EIEC invasion and reduced the secretion of proinflammatory cytokines.CONCLUSION: Bifico reduced colon inflammation in IL-10 KO mice, and promoted and improved epithelialbarrier function, enhanced resistance to EIEC invasion, and decreased proinflammatory cytokine secretion. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved.