A Genomewide Survey Argues That Every Zygotic Gene Product Is Dispensable for the Initiation of Somatic Homolog Pairing in Drosophila
A Genomewide Survey Argues That Every Zygotic Gene Product Is Dispensable for the Initiation of Somatic Homolog Pairing in Drosophila
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DOI：
10.1534/genetics.108.094862
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发表时间：
2008-11-01
期刊：
影响因子：
3.3
通讯作者：
Wu, C. -ting
中科院分区：
文献类型：
--
作者：
Bateman, Jack R.;Wu, C. -ting
对不同生物体的研究表明，不同的染色体间相互作用与许多发育事件有关。尽管最近在发现这些现象方面取得了进展，但我们对染色体间相互作用如何启动和调节的理解是不完整的。在果蝇胚胎发生的母系-合子转换（MZT）过程中，母系和父系同源染色体之间形成稳定的染色体间接触，这种现象称为体细胞同源配对。为了更好地理解启动配对的事件，我们对合子对这一过程的贡献进行了全基因组评估。具体地说，我们利用复合染色体的分离特性来产生缺乏整个染色体臂的胚胎，因此，所有合子基因产物都来自这些臂。使用DNA荧光原位杂交（FISH）来评估在五个不同的位点配对的开始，这种方法使我们能够调查整个合子基因组仅使用少数的十字架。值得注意的是，我们没有发现任何缺陷的配对胚胎缺乏任何染色体臂，表明没有合子基因产物是必不可少的配对开始。从这些数据中，我们得出结论，配对的启动可以独立于合子控制，因此可能是由母体基因组编码的发育程序的一部分。
Studies from diverse organisms show that distinct interchromosomal interactions are associated with many developmental events. Despite recent advances in uncovering such phenomena, our understanding of how interchromosomal interactions are initiated and regulated is incomplete. During the maternal-to-zygotic transition (MZT) of Drosophila embryogenesis, stable interchromosomal contacts form between maternal and paternal homologous chromosomes, a phenomenon known its somatic homolog pairing. To better understand the events that initiate pairing, we performed a genomewide assessment of the zygotic contribution to this process. Specifically, we took advantage of the segregational properties of compound chromosomes to generate embryos lacking entire chromosome arms and, thus, all zygotic gene products derived from those arms. Using DNA fluorescence in situ hybridization (FISH) to assess the initiation of pairing at five separate loci, this approach allowed us to survey the entire zygotic genome using just a handful of crosses. Remarkably, we found no defect in pairing in embryos lacking any chromosome arm, indicating that no zygotic gene product is essential for pairing to initiate. From these data, we conclude that the initiation of pairing can occur independently of zygotic control and may therefore be part of the developmental program encoded by the maternal genome.