Interaction of the replication proteins and the capsid protein of porcine circovirus type 1 and 2 with host proteins
Interaction of the replication proteins and the capsid protein of porcine circovirus type 1 and 2 with host proteins
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DOI：
10.1016/j.virol.2008.12.039
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发表时间：
2009-03-30
期刊：
影响因子：
3.7
通讯作者：
Mankertz, Annette
中科院分区：
文献类型：
--
作者：
Finsterbusch, Tim;Steinfeldt, Tobias;Mankertz, Annette
猪圆环病毒2型（Porcine Circovirus type 2，PCV 2）是猪的重要病原体，而猪圆环病毒1型（Porcine Circovirus type 1，PCV 1）是猪的非致病性病毒。为了分析PCV与其宿主之间的相互作用，我们使用酵母双杂交测定来鉴定与两种PCV基因型的Cap和Rep蛋白相互作用的细胞蛋白。发现六种细胞蛋白与Cap相互作用（MKRN 1，gC 1 qR，Par-4。NAP 1、NPM 1和Hsp 40）和三种Rep（ZNF 265、TDG和VG 5 Q）。通过免疫共沉淀证实了这些相互作用。通过免疫荧光对蛋白质的定位的调查显示，在某些情况下，仅与Cap有限的空间重叠，而在其他情况下，观察到明显的共定位和显著的蛋白质再分布。这九种细胞蛋白与病毒生命周期的不同方面相关，我们的数据可能支持PCV 2发病机制领域的未来研究。(c)2009 Elsevier Inc. All rights reserved.
Porcine circovirus type 2 (PCV2) is an important pathogen in swine, whereas porcine circovirus type 1 (PCV1) is apathogenic. To analyze the interactions between PCV and its host, we have used a yeast two-hybrid assay to identify cellular proteins interacting with Cap and Rep proteins of both PCV genotypes. Six cellular proteins were found to interact with Cap (MKRN1, gC1qR, Par-4. NAP1, NPM1 and Hsp40) and three with Rep (ZNF265, TDG and VG5Q). These interactions were confirmed by co-immunoprecipitation. Investigation of the localisation of the proteins by immunofluorescence revealed in some cases only limited spatial overlapping with Cap, while in others a clear co-localisation and prominent protein redistribution was observed. The nine cellular proteins are associated with distinct aspects of viral lifecycle and our data is likely to support future research in the field of PCV2 pathogenesis. (c) 2009 Elsevier Inc. All rights reserved.