Yokukansan, a traditional Japanese herbal medicine, alleviates the emotional abnormality induced by maladaptation to stress in mice
Yokukansan, a traditional Japanese herbal medicine, alleviates the emotional abnormality induced by maladaptation to stress in mice
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DOI：
10.1016/j.phymed.2013.08.025
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发表时间：
2014-02-15
期刊：
影响因子：
7.9
通讯作者：
Takeda, Hiroshi
中科院分区：
文献类型：
--
作者：
Tsuji, Minoru;Takeuchi, Tomoko;Takeda, Hiroshi
本研究的目的是探讨抑肝散（由苍术、茯苓、蛇床子、钩藤、当归、柴胡和甘草组成的日本传统草药）对小鼠压力适应不良引起的情绪异常的影响。小鼠每天受到 60 或 240 分钟的重复束缚压力，持续 14 天。从应激暴露的第 3 天开始，在每天暴露于束缚应激后立即给小鼠口服 yokukansan (p.o.) 或腹膜内 (i.p.) 5-HT1A 受体激动剂 flesinoxan。最终暴露于束缚压力后，使用自动孔板装置评估小鼠的情绪。在孔板测试中，单次暴露于约束应力 60 分钟会导致低头行为减少。在持续 14 天、每天 60 分钟的重复束缚压力下，这种情绪压力反应消失了，这证实了压力适应的发展。相比之下，持续 14 天、每天 240 分钟的束缚压力的小鼠并没有形成这种压力适应能力，并且低头行为仍然有所减少。在压力适应不良的小鼠中观察到的情绪下降在每日暴露于压力后立即使用yokukansan（1000 mg/kg，口服）以及氟辛克生（0.25和0.5 mg/kg，腹腔注射）进行长期治疗后显着恢复。这些发现表明，抑肝散可能对压力适应有有益的作用，可以缓解过度压力条件下的情绪异常。 (C) 2013 爱思唯尔有限公司。版权所有。
The aim of the present study was to examine the effect of yokukansan, a traditional Japanese herbal medicine that is composed of Atractylodis lanceae Rhizoma, Poria, Cnidii Rhizoma, Uncariae Uncis cum Ramulus, Angelicae Radix, Bupleuri Radix and Glycyrrhizae Radix, on the emotional abnormality induced by maladaptation to stress in mice. Mice were exposed to repeated restraint stress for 60 or 240 min/day for 14 days. From the 3rd day of stress exposure, mice were given yokukansan orally (p.o.) or the 5-HT1A receptor agonist flesinoxan intraperitoneally (i.p.) immediately after the daily exposure to restraint stress. After the final exposure to restraint stress, the emotionality of mice was evaluated using an automatic hole-board apparatus. A single exposure to restraint stress for 60 min induced a decrease in head-dipping behavior in the hole-board test. This emotional stress response disappeared in mice that had been exposed to repeated restraint stress for 60 min/day for 14 days, which confirmed the development of stress adaptation. In contrast, mice that were exposed to restraint stress for 240 min/day for 14 days did not develop this stress adaptation, and still showed a decrease in head-dipping behavior. The decreased emotionality observed in stress-maladaptive mice was significantly recovered by chronic treatment with yokukansan (1000 mg/kg, p.o.) as well as flesinoxan (0.25 and 0.5 mg/kg, i.p.) immediately after daily exposure to stress. These findings suggest that yokukansan may have a beneficial effect on stress adaptation and alleviate the emotional abnormality under conditions of excessive stress. (C) 2013 Elsevier GmbH. All rights reserved.