Effects of insulin-like growth factor-I during early postnatal period in intrauterine growth-restricted rats.
Effects of insulin-like growth factor-I during early postnatal period in intrauterine growth-restricted rats.
复制标题
胰岛素样生长因子-I 对宫内生长受限大鼠产后早期的影响。
DOI：
10.1111/ped.12855
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发表时间：
2015
影响因子：
1.4
通讯作者：
Toshiaki Shimizu
中科院分区：
文献类型：
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作者：
Naho Ikeda;Hiromichi Shoji;Toshiaki Shimizu
背景胰岛素样生长因子-I (IGF-I) 对于围产期生长和发育至关重要；在宫内生长受限 (IUGR) 期间观察到血清 IGF-I 较低。我们研究了重组人（rh）IGF-I对IUGR大鼠产后早期的影响。方法通过结扎妊娠大鼠双侧子宫动脉诱导宫内生长受限。 IUGR幼崽被分为两组，从出生后第7天（PND）到第13天，每天注射rhIGF-I（2mg/kg；IUGR/IGF-I，n = 16）或盐水（IUGR/生理盐水溶液（PSS），n = 16）。母体假手术幼崽注射盐水作为对照（对照， n = 16）。在 PND25 上测量血清 IGF-I 和 IGF 结合蛋白 (IGFBP) 3 和 5。使用 PND25 上的实时聚合酶链反应测量肝脏和大脑中 Igf-i、IGF-I 受体 (Igf-ir)、Igfbp3 和 5mRNA 的表达。对肝脏进行免疫组织化学染色以检测 IGF 表达。结果 IUGR 幼犬（IUGR/IGF-I 和 IUGR/PSS）中 PND3 和 PND25 的平均体重显着低于对照幼犬。 IUGR 幼仔的血清 IGF-I 和肝脏 Igf-irmRNA 显着低于对照幼仔。在IUGR/IGF-I组中，肝脏Igfbp3mRNA和肝脏免疫组织化学染色增加。在 IUGR/PSS 和对照幼崽中，这两组之间的血清 IGFBP3 和 IGFBP5、肝脏 Igf-iandIgfbp-5 mRNA 或脑 IgfmRNA 没有显着差异。 结论 在产后早期使用 2mg/kg rhIGF-I 治疗后，观察到对身体和大脑体重增加没有益处，但肝脏 IGFBP-3 有效增加 时期。
BackgroundInsulin‐like growth factor‐I (IGF‐I) is essential for perinatal growth and development; low serum IGF‐I has been observed during intrauterine growth restriction (IUGR). We investigated the effects of recombinant human (rh) IGF‐I in IUGR rats during the early postnatal period.MethodsIntrauterine growth restriction was induced by bilateral uterine artery ligation in pregnant rats. IUGR pups were divided into two groups injected daily with rhIGF‐I (2 mg/kg; IUGR/IGF‐I, n = 16) or saline (IUGR/physiologic saline solution (PSS), n = 16) from postnatal day (PND) 7 to 13. Maternal sham‐operated pups injected with saline were used as controls (control, n = 16). Serum IGF‐I and IGF binding proteins (IGFBP) 3 and 5 were measured on PND25. The expression ofIgf‐i, IGF‐I receptor (Igf‐ir),Igfbp3, and5mRNA in the liver and brain was measured using real‐time polymerase chain reaction on PND25. Immunohistochemical staining of the liver for IGF expression was performed.ResultsMean bodyweight on PND3 and PND25 in the IUGR pups (IUGR/IGF‐I and IUGR/PSS) was significantly lower than that of the control pups. Serum IGF‐I and hepaticIgf‐irmRNA in the IUGR pups were significantly lower than those in the control pups. In the IUGR/IGF‐I group, hepaticIgfbp3mRNA and liver immunohistochemical staining were increased. In the IUGR/PSS and control pups, there were no significant differences between these two groups in serum IGFBP3 and IGFBP5, hepaticIgf‐iandIgfbp‐5mRNA, or brainIgfmRNA.ConclusionsNo benefits on body and brain weight gain but an effective increase in hepatic IGFBP‐3 was observed after treatment with 2 mg/kg rhIGF‐I during the early postnatal period.