Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5' AMP-activated protein kinase (AMPK)
Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5' AMP-activated protein kinase (AMPK)
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DOI：
10.1016/j.cryobiol.2014.10.008
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发表时间：
2014-12-01
期刊：
影响因子：
2.7
通讯作者：
Ramirez-Reveco, Alfredo
中科院分区：
文献类型：
--
作者：
Cordova, Alex;Strobel, Pablo;Ramirez-Reveco, Alfredo
本研究评估了在AMPK激活剂存在或不存在的情况下使用低代谢TRIS扩展剂以及在冷藏步骤中使用高冷却速率对种马精子冷冻性的影响。用Botucrio(R)作为对照扩展剂和基础TRIS扩展剂（HM-0）分别添加10 mM二甲双胍、2 mM 5-氨基咪唑-4-羧酰胺-1- β - d -核呋喃苷（AICAR）、2 mM单磷酸腺苷（AMP）、我们的研究结果表明，与商用扩展剂相比，使用添加或不添加AMP或二甲双胍的低代谢TRIS扩展剂显著提高了种马精子的冷冻性。此外，高冷却速率不会影响冷却和解冻后的种马精子质量。最后，种马精子呈现出几种假定的AMPK精子同工型，它们似乎对经典的激活剂没有反应，但对化合物C抑制剂有反应。(C) 2014爱思唯尔公司版权所有。
This study evaluated the effect of the use of hypometabolic TRIS extenders in the presence or the absence of AMPK activators as well as the utilization of high cooling rates in the refrigeration step on the freezability of stallion sperm. Twelve ejaculates were cryopreserved using Botucrio(R) as a control extender and a basic TRIS extender (HM-0) separately supplemented with 10 mM metformin, 2 mM 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), 2 mM Adenosine monophosphate (AMP), 40 mu M compound C AMPK inhibitor or 2 mM AMP + 40 mu M compound C. Our results showed that the utilization of a hypometabolic TRIS extender supplemented or not with AMP or metformin significantly improves stallion sperm freezability when compared with a commercial extender. Additionally, high cooling rates do not affect stallion sperm quality after cooling and post-thawing. Finally, stallion spermatozoa present several putative AMPK sperm isoforms that do not seem to respond to classical activators, but do respond to the Compound C inhibitor. (C) 2014 Elsevier Inc. All rights reserved.