Electrochemical Nanoprobes for Single-Cell Analysis
Electrochemical Nanoprobes for Single-Cell Analysis
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DOI：
10.1021/nn405612q
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发表时间：
2014-01-01
期刊：
影响因子：
17.1
通讯作者：
Korchev, Yuri E.
中科院分区：
文献类型：
--
作者：
Actis, Paolo;Tokar, Sergiy;Korchev, Yuri E.
在对生物环境破坏最小的情况下测量单个细胞中的关键分子是单细胞分析的下一个前沿。纳米尺度设备是理想的分析工具，因为它们的尺寸小并且具有高空间和时间分辨率记录的潜力。在这里，我们报告了盘形碳纳米电极的制造，其半径可以在5-200 nm范围内精确调谐。用铂对纳米电极进行功能化，可以监测脑切片内外的耗氧量。此外，我们表明，这种类型的纳米电极可用于刺穿单个细胞，以在细胞内进行电化学测量，对细胞功能的破坏最小。这些纳米电极可以与扫描离子电导显微镜探针结合制造，这应该允许活细胞上或活细胞中的物种的高分辨率电化学映射。
The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.