Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis.
Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis.
复制标题
通过 ELISA 和免疫印迹分析狗血清对伯氏疏螺旋体全细胞或重组抗原的反应性。
DOI：
10.1099/0022-1317-50-10-889
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发表时间：
2001
影响因子：
3
通讯作者：
Fikrig,Erol
中科院分区：
文献类型：
--
作者：
Magnarelli,LouisA;Levy,StevenA;Ijdo,JacobW;Wu,Caiyun;Padula,StevenJ;Fikrig,Erol
采用酶联免疫吸附法（elisa）分别制备10种纯化的严格感伯氏疏螺旋体重组抗原，对36只未接种该菌全细胞的犬和5只接种了伯氏疏螺旋体全细胞的犬的血清进行检测。所有的狗都生活在美国康涅狄格州和纽约州东南部蜱虫肆虐的地区。未接种疫苗的狗在1984-1991年期间有肢体或关节紊乱、跛行和发烧，并有b抗体。用全细胞抗原多价ELISA检测伯氏疏螺旋体。在用重组抗原重新分析血清总免疫球蛋白时，分别检测外表面蛋白（Osp） F、蛋白(p)35、p37、p39和p- 41g（鞭毛蛋白成分）时，反应最频繁。16份经ELISA检测全细胞抗原呈阳性且抗体滴度范围（640-40 - 960）的血清的Western免疫印迹证实对这些或其他裂解的全细胞抗原有免疫应答。接种疫苗犬血清中含有OspA、OspB、p22、p37和p41-G抗体。因此，对OspF、p35和p39的血清学反应是b .burgdorferi自然暴露的最重要指标。在elisa中，血清对这些重组抗原的反应性可用于帮助鉴定未接种全细胞b疫苗的犬可能的犬螺旋体病自然感染。Burgdorferiand提供了这种细菌的地理分布信息。
Enzyme-linked immunosorbent assays (ELISAs) with separate preparations of 10 purified recombinant antigens ofBorrelia burgdorferi sensu strictowere used to test sera from 36 dogs not vaccinated with whole cells of this agent and from five dogs vaccinated with whole-cellB.burgdorferibacteria. All dogs lived in tick-infested areas of Connecticut and south-eastern New York state, USA. The non-vaccinated dogs had limb or joint disorder, lameness and fever during the period 1984–1991 and had antibodies toB. burgdorferi, as determined by a polyvalent ELISA with whole-cell antigen. In re-analyses of sera for total immunoglobulins in ELISAs with recombinant antigens, reactions were most frequently recorded when outer-surface protein (Osp) F, protein (p)35, p37, p39 and p-41G (a flagellin component) were tested separately. Western immunoblots of a subset of 16 sera, positive by ELISA with whole-cell antigen and representing a range of antibody titres (640–40 960), verified immune responses to these or other lysed whole-cell antigens. Sera from vaccinated dogs contained antibodies to OspA, OspB, p22, p37 and p41-G. Therefore, serological reactions to OspF, p35 and p39 were the most important indicators of natural exposure toB.burgdorferi. Serum reactivities to these recombinant antigens in ELISAs can be used to help identify possible natural infections of canine borreliosis in dogs not vaccinated with whole-cellB.burgdorferiand to provide information on the geographic distribution of this bacterium.