DEVELOPMENT OF MEASLES VIRUS IN VITRO
DEVELOPMENT OF MEASLES VIRUS IN VITRO
复制标题
DOI：
10.1016/0042-6822(69)90127-5
复制
发表时间：
1969-01-01
期刊：
影响因子：
3.7
通讯作者：
HOWATSON, AF
中科院分区：
文献类型：
--
作者：
NAKAI, T;SHAND, FL;HOWATSON, AF
用电镜技术研究了麻疹病毒在体外培养细胞中的发育顺序。观察结果与传染性试验测定的病毒生长有关。首先在感染细胞的细胞质中检测到病毒核蛋白小管，不久之后在细胞核中检测到类似的小管。在任何一个细胞中，小管通常存在于细胞核或细胞质中，很少同时存在于两者。没有证据表明小管从一个部位迁移到另一个部位。在感染周期的后期，细胞质小管在细胞表面的出芽过程中被挤出，导致形成包膜颗粒，这被解释为麻疹病毒粒子。细胞质小管形成与活跃的出芽和高产量有关，而当核内形成占优势时，很少或不检测到出芽，病毒产量低。阴性染色的核衣壳长度分布在1.4 ~ 1.6 μ处出现峰值。当考虑到核衣壳螺旋的拉伸时，这个数字降低到1.1 μ。每个核衣壳的螺旋转数达到峰值，为204。假设麻疹病毒核衣壳的构象与烟草花叶病毒相似，计算出麻疹病毒RNA的长度为6.4μ，分子量为6.2 × 106道尔顿。
The sequence of development of measles virus in cells cultivatedin vitrowas studied by electron microscopic techniques. The observations were correlated with virus growth measured by infectivity assay. Viral nucleoprotein tubules were first detected in the cytoplasm of infected cells, and shortly thereafter similar tubules were detected in the nucleus. In any one cell tubules were usually present either in the nucleus or in the cytoplasm, and rarely in both. No evidence suggesting that tubules migrated from one site to the other was obtained. At later stages in the infectious cycle, cytoplasmic tubules were extruded by a budding process at the cell surface, resulting in the formation of enveloped particles, interpreted as measles virions. Cytoplasmic tubule formation was associated with active budding and high yields of virus, whereas when intranuclear formation was predominant, little or no budding was detected and viral yields were low.The distribution of lengths of isolated nucleocapsids in negatively stained preparations showed a peak at 1.4-1.6 μ. When allowance was made for stretching of the nucleocapsid helix, this figure was reduced to 1.1 μ. The number of helical turns per nucleocapsid peaked sharply at a value of 204. On the assumption that the conformation of the measles nucleocapsid is similar to that of tobacco mosaic virus, the length and molecular weight of measles virus RNA were calculated and found to be 6.4μ and 6.2 × 106daltons, respectively.