pH, redox potential and local biofilm potential microenvironments within Geobacter sulfurreducens biofilms and their roles in electron transfer.
pH, redox potential and local biofilm potential microenvironments within Geobacter sulfurreducens biofilms and their roles in electron transfer.
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DOI：
10.1002/bit.24538
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发表时间：
2012-10
影响因子：
3.8
通讯作者：
Beyenal, Haluk
中科院分区：
文献类型：
--
作者：
Babauta, Jerome T.;Hung Duc Nguyen;Harrington, Timothy D.;Renslow, Ryan;Beyenal, Haluk
关键词：
电极呼吸生物膜内pH的限制是一个众所周知的概念。然而，对于电极上呼吸的生物膜内电流的增加如何影响pH和氧化还原电位，人们知之甚少。需要量化pH和氧化还原电位随着电流的增加而变化，以确定电子转移如何与生物膜内的质子转移联系在一起。在这项研究中，我们量化了电极呼吸硫还原地杆菌生物膜中pH和氧化还原电位的变化，作为呼吸速率的函数，以电流测量。我们还表征了对电极上的pH和氧化还原电位。我们的结论是：(1)生物膜中的pH在不同的生长阶段持续下降，表明pH并不总是生物膜中的限制因素；(2)生物膜电极上pH的降低和氧化还原电位的增加只与生物膜有关，表明硫还原菌生物膜在独特的内部环境中呼吸。还将生物膜内的氧化还原电位与石墨微电极测量的局部生物膜电位进行了比较，在石墨微电极的尖端被允许在生物膜内驯化。
The limitation of pH inside electrode-respiring biofilms is a well-known concept. However, little is known about how pH and redox potential are affected by increasing current inside biofilms respiring on electrodes. Quantifying the variations in pH and redox potential with increasing current is needed to determine how electron transfer is tied to proton transfer within the biofilm. In this research, we quantified pH and redox potential variations in electrode-respiring Geobacter sulfurreducens biofilms as a function of respiration rates, measured as current. We also characterized pH and redox potential at the counter electrode. We concluded that (1) pH continued to decrease in the biofilm through different growth phases, showing that the pH is not always a limiting factor in a biofilm and (2) decreasing pH and increasing redox potential at the biofilm electrode were associated only with the biofilm, demonstrating that G. sulfurreducens biofilms respire in a unique internal environment. Redox potential inside the biofilm was also compared to the local biofilm potential measured by a graphite microelectrode, where the tip of the micro-electrode was allowed to acclimatize inside the biofilm.
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影响因子：
4.4
作者：
Kroukamp, Otini;Wolfaardt, Gideon M.
通讯作者：
Wolfaardt, Gideon M.
影响因子：
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Nevin KP;Kim BC;Glaven RH;Johnson JP;Woodard TL;Methé BA;Didonato RJ;Covalla SF;Franks AE;Liu A;Lovley DR
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影响因子：
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影响因子：
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作者：
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通讯作者：
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