G protein-activated K+ channels: a reporter for rapid activation of G proteins by lysophosphatidic acid in Xenopus oocytes.
G protein-activated K+ channels: a reporter for rapid activation of G proteins by lysophosphatidic acid in Xenopus oocytes.
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G 蛋白激活的 K 通道：爪蟾卵母细胞中溶血磷脂酸快速激活 G 蛋白的报告基因。
DOI：
10.1016/s0014-5793(04)00334-5
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发表时间：
2004
期刊：
影响因子：
3.5
通讯作者：
Oron,Yoram
中科院分区：
文献类型：
--
作者：
ItzhakiVan-Ham,Irit;Peleg,Sagit;Dascal,Nathan;Shapira,Hagit;Oron,Yoram
溶血磷脂酸（LPA）或乙酰胆碱（ACh）的阈值浓度诱导百日咳毒素（PTX）敏感性反应的快速脱敏对LPA在异种卵母细胞。为了证明阈值[LPA]快速激活Gi/o蛋白，我们使用G蛋白激活的K+通道（GIRK）作为报告基因。低[LPA]在<3 s的激动剂添加中诱导IK+，几乎没有或没有激活氯电流。Gαo/Gα o 1的耗竭使LPA-诱导的IK+减少约40- 50%，而PTX完全消除了它。这是第一个直接证据表明LPA激活GIRK，Go家族的G蛋白参与LPA反应的快速脱敏。
Threshold concentrations of lysophosphatidic acid (LPA) or acetylcholine (ACh) induce pertussis toxin (PTX)‐sensitive rapid desensitization of responses to LPA inXenopusoocytes. To demonstrate that threshold [LPA] rapidly activates Gi/o proteins, we used the G protein‐activated K+channel (GIRK) as a reporter. Low [LPA] inducedIK+in <3 s of the agonist addition with little or no activation of chloride current. Depletion of Gαo/Gαo1 each decreased the LPA‐inducedIK+by approximately 40–50%, while PTX completely abolished it. This is the first direct evidence showing the activation of GIRK by LPA, and the involvement of G proteins of the Go family in rapid desensitization of LPA responses.