ELECTRICAL-RESISTANCE MEASUREMENTS ON CEREBRAL CAPILLARY ENDOTHELIAL-CELLS - A NEW TECHNIQUE TO STUDY SMALL SURFACE-AREAS
ELECTRICAL-RESISTANCE MEASUREMENTS ON CEREBRAL CAPILLARY ENDOTHELIAL-CELLS - A NEW TECHNIQUE TO STUDY SMALL SURFACE-AREAS
复制标题
DOI：
10.1016/0165-022x(95)00011-x
复制
发表时间：
1995-11-01
影响因子：
--
通讯作者：
GALLA, HJ
中科院分区：
文献类型：
--
作者：
ERBEN, M;DECKER, S;GALLA, HJ
我们提出了一种新的方法，允许在尺寸小于1毫米(2)的选定细胞单层区域进行电阻测量。到目前为止，只有在不存在不均匀性或污染细胞的情况下，使用面积达5厘米(2)的覆盖细胞的宏观过滤器才能给出可靠的结果。新的测量装置放置在光学显微镜上，可以对测量区域进行光学表征，并使我们能够扫描细胞覆盖的滤波器。这种方法已被应用于确定在培养上皮细胞或内皮细胞之间的细胞间接触紧密度的可逆调节。猪脑微血管内皮细胞（血脑屏障）的紧密连接阻力是由Ca2+和碱性氨基酸调节的。
We present a new method which allows resistance measurements in selected cell monolayer areas with a size of less than one mm(2). Up to now cell-covered macroscopic filters with areas up to 5 cm(2) have been used giving reliable results only in the absence of inhomogeneities or contaminating cells. The new measuring device is posed on the microscope optic to allow an optical characterization of the measuring area and enables us to scan the cell-covered filter. This method has been applied to determine a reversible modulation of the tightness of intercellular contacts between epithelial or endothelial cells in culture. Tight junction resistance is modulated by Ca2+ and basic amino acids in cultured porcine cerebral microvascular endothelial cells that represent the blood-brain barrier.