Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy
Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy
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DOI：
10.1016/j.colsurfb.2011.05.044
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发表时间：
2011-11-01
影响因子：
5.8
通讯作者：
Landes, Christy F.
中科院分区：
文献类型：
--
作者：
Daniels, Charlisa R.;Reznik, Carmen;Landes, Christy F.
本文报道了原位观察有机染料探针分子和染料标记蛋白质与不同聚乙二醇(PEG)结构(线状、树枝状和瓶刷)的相互作用。利用荧光相关光谱(FCS)和单分子事件分析研究了探针与聚乙二醇单分子相互作用的性质和程度。这些数据支持筛状模型，在该模型中，大小排除原则决定了探针-聚乙二醇相互作用的程度。较小的探针被密度较高的聚乙二醇组分捕获，而较大的探针与密度较低的聚乙二醇表面更容易相互作用。这些结果，以及这项工作中使用的聚乙二醇树枝的可调孔结构，表明了用于可调表面的电化学活性材料的可行性。(C)2011爱思唯尔B.V.保留所有权利。
The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces. (C) 2011 Elsevier B.V. All rights reserved.