Angiotensin II stimulates the proliferation and biosynthesis of type I collagen in cultured murine mesangial cells.
Angiotensin II stimulates the proliferation and biosynthesis of type I collagen in cultured murine mesangial cells.
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发表时间：
1992
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通讯作者：
G. Wolf;U. Haberstroh;E. Neilson
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作者：
G. Wolf;U. Haberstroh;E. Neilson
从SJL小鼠肾小球中建立了小鼠系膜细胞系（MMC），以研究血管紧张素Ⅱ（ANG Ⅱ）对无血清培养的MMC生长和功能的影响。小鼠系膜细胞表现出系膜细胞的表型特征，包括免疫荧光染色结蛋白、波形蛋白、Thy 1以及I型和IV型胶原。在无血清培养液中，每日加入10（-6）~ 10（-11）mol/l的ANG Ⅱ也能诱导MMC增殖。这种对生长的影响独立于培养基中胰岛素的存在，并且是受体介导的，因为特异性ANG II受体拮抗剂DuP 753废除了增殖性生长。血管紧张素II也刺激主要是在我们的MMCs的I型胶原的生物合成。用含有α 2（I）和α 1（IV）胶原的增强子/启动子元件的嵌合基因转染MMC，该元件与氯霉素乙酰转移酶报告基因连接，表明ANG II对I型胶原的刺激作用至少在一定程度上取决于转录的增加。这些研究结果共同表明，ANG II在无血清培养可以是一个旁分泌催化剂的生长和I型胶原在系膜细胞的生物合成。
A murine mesangial cell line (MMC) was established from the glomeruli of SJL mice to study the influence of angiotensin II (ANG II) on their growth and function in a serum-free culture. Murine mesangial cells exhibit the phenotypic characteristics of mesangial cells, including staining for desmin, vimentin, Thy 1, and types I and IV collagen by immunofluorescence. The addition of daily doses of 10(-6) to 10(-11) mol/l ANG II to MMCs also induced their proliferation in serum-free media. This effect on growth was independent of the presence of insulin in the media, and was receptor mediated, because the specific ANG II-receptor antagonist DuP 753 abolished proliferative growth. Angiotensin II also stimulated mainly the biosynthesis of type I collagen in our MMCs. Transfection of MMCs with chimeric genes containing enhancer/promoter elements for alpha 2(I) and alpha 1(IV) collagens linked to a chloramphenicol acetyltransferase reporter demonstrated that the stimulatory effect of ANG II for type I depends, at least to some extent, on an increase in transcription. These findings indicate collectively that ANG II in serum-free cultures can be a paracrine catalyst for the growth and biosynthesis of type I collagen in mesangial cells.