A Biodegradable and Biocompatible Regular Nanopattern for Large-Scale Selective Cell Growth
A Biodegradable and Biocompatible Regular Nanopattern for Large-Scale Selective Cell Growth
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DOI：
10.1002/smll.201000193
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发表时间：
2010-12-06
期刊：
影响因子：
13.3
通讯作者：
Riehle, Mathis O.
中科院分区：
文献类型：
--
作者：
Csaderova, Lucia;Martines, Elena;Riehle, Mathis O.
通过电子束光刻和热压印制造的具有规则纳米柱阵列的可生物降解基底用于解决细胞行为的纳米形貌控制机制。在纳米柱上培养的两种不同的细胞系显示出细胞覆盖率的显着差异。这些变化取决于形貌和细胞，并且不是由纳米图案上捕获的气泡介导的。首次在大面积上清楚地证明了相同纳米形貌的强细胞选择性效应；虽然成纤维细胞增殖受到抑制，但内皮细胞的扩散明显增强。成纤维细胞增殖的减少表明纳米形貌引起的可用表面积的减少可能是影响纳米图案上细胞生长的主要因素。本文提出的结果为永久性血管替代物的开发铺平了道路，其中非粘性、惰性表面将诱导快速原位内皮化，以减少血栓形成和闭塞。
A biodegradable substrate with a regular array of nanopillars fabricated by electron-beam lithography and hot embossing is used to address the mechanisms of nanotopographical control of cell behavior. Two different cell lines cultured on the nanopillars show striking differences in cell coverage. These changes are topography-and cell-dependent, and are not mediated by air bubbles trapped on the nanopattern. For the first time, a strong cell-selective effect of the same nanotopography has been clearly demonstrated on a large area; while fibroblast proliferation is inhibited, endothelial cell spreading is visibly enhanced. The reduced fibroblast proliferation indicates that a reduction of available surface area induced by nanotopography might be the main factor affecting cell growth on nanopatterns. The results presented herein pave the way towards the development of permanent vascular replacements, where non-adhesive, inert, surfaces will induce rapid in situ endothelialization to reduce thrombosis and occlusion.