Hydrogen exchange and mass spectrometry: A historical perspective
Hydrogen exchange and mass spectrometry: A historical perspective
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DOI：
10.1016/j.jasms.2006.06.006
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发表时间：
2006-11-01
影响因子：
3.2
通讯作者：
Englander, S. Walter
中科院分区：
文献类型：
--
作者：
Englander, S. Walter
蛋白质分子用氢交换的语言自然地发出信息丰富的信号流，涉及它们的稳定性、动力学、功能、其中的变化及其影响的亲密细节，所有这些都被分解到单个氨基酸的水平。测量蛋白质氢交换行为，了解氢交换过程的潜在化学和结构物理，并利用这些信息来了解蛋白质的性质和功能的努力已经持续了50年。最近的工作使用质谱分析和早期的蛋白质水解碎裂方法将氢交换能力扩展到大型生物感兴趣的蛋白质。本文简要回顾了导致我们达到这一点的进展以及迄今为止所取得的理解。
Protein molecules naturally emit streams of information-rich signals in the language of hydrogen exchange concerning the intimate details of their stability, dynamics, function, changes therein, and effects thereon, all resolved to the level of their individual amino acids. The effort to measure protein hydrogen exchange behavior, understand the underlying chemistry and structural physics of hydrogen exchange processes, and use this information to learn about protein properties and function has continued for 50 years. Recent work uses mass spectrometric analysis together with an earlier proteolytic fragmentation method to extend the hydrogen exchange capability to large biologically interesting proteins. This article briefly reviews the advances that have led us to this point and the understanding that has so far been achieved.