Comparison of Bolus and Infusion Methods for Receptor Quantitation: Application to [18F]Cyclofoxy and Positron Emission Tomography
Comparison of Bolus and Infusion Methods for Receptor Quantitation: Application to [18F]Cyclofoxy and Positron Emission Tomography
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用于受体定量的推注和输注方法的比较：在 [18F]Cyclofoxy 和正电子发射断层扫描中的应用
DOI：
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发表时间：
1993
影响因子：
6.3
通讯作者：
P. Herscovitch
中科院分区：
文献类型：
--
作者：
Richard E. Carson;M. Channing;Ronald G. Blasberg;B. Dunn;Robert M. Cohen;K. Rice;P. Herscovitch
在狒狒中进行了阿片拮抗剂[18 F]cyclofoxy（[18 F]CF）的正电子发射断层扫描研究。推注研究表明初始摄取依赖于血流。晚期摄取显示尾状核、杏仁核、丘脑和脑干中的结合最高，小脑中的蓄积最少。注射后60分钟，局部脑放射性以与代谢物校正血浆相同的速率清除，即，实现了瞬态平衡。将房室建模方法应用于脑和代谢物校正血浆的时间-活性曲线。个别速率常数估计精度差。可靠地确定了总分布容积（VT）的模型估计值，代表平衡时组织放射性与代谢物校正血浆的比值。将表观分布容积（Va）（瞬时平衡期间组织与代谢物校正血浆的浓度比）与拟合VT值进行比较，以确定单次扫描方法是否可以提供准确的受体测量值。Va明显高估VT，并产生人为的高图像对比度。这些差异是通过房室模型理论预测的，是由血浆清除率接近最慢的组织清除率引起的。为了开发一种测量VT的简单方法，设计了一种由CF的推注加连续输注（B/I）组成的输注方案，并应用于一组单独的研究。B/I研究的Va值与B/I和推注研究的VT值一致。这种输注方法可以产生准确的受体测量，并有可能缩短扫描时间，简化扫描和血液数据的采集和处理。
Positron emission tomography studies with the opiate antagonist [18F]cyclofoxy ([18F]CF) were performed in baboons. Bolus injection studies demonstrated initial uptake dependent on blood flow. The late uptake showed highest binding in caudate nuclei, amygdala, thalamus, and brainstem and the least accumulation in cerebellum. By 60 min postinjection, regional brain radioactivity cleared at the same rate as metabolite-corrected plasma, i.e., transient equilibrium was achieved. Compartmental modeling methods were applied to time-activity curves from brain and metabolite-corrected plasma. Individual rate constants were estimated with poor precision. The model estimate of the total volume of distribution (VT), representing the ratio of tissue radioactivity to metabolite-corrected plasma at equilibrium, was reliably determined. The apparent volume of distribution (Va), the concentration ratio of tissue to metabolite-corrected plasma during transient equilibrium, was compared with the fitted VT values to determine if single-scan methods could provide accurate receptor measurements. Va significantly overestimated VT and produced artificially high image contrast. These differences were predicted by compartment model theory and were caused by a plasma clearance rate that was close to the slowest tissue clearance rate. To develop a simple method to measure VT, an infusion protocol consisting of bolus plus continuous infusion (B/I) of CF was designed and applied in a separate set of studies. The Va values from the B/I studies agreed with the VT values from both B/I and bolus studies. This infusion approach can produce accurate receptor measurements and has the potential to shorten scan time and simplify the acquisition and processing of scan and blood data.
定量体内受体结合 III：毒蕈碱胆碱能受体结合的示踪动力学模型。
DOI：
10.1073/pnas.82.19.6711
发表时间：
1985
影响因子：
11.1
作者：
Frey,KA;Hichwa,RD;Ehrenkaufer,RL;Agranoff,BW
通讯作者：
Agranoff,BW
使用放射性标记受体配体对神经递质系统进行体内成像。
DOI：
10.1016/s0893-133x(97)00069-9
发表时间：
1997
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
Kegeles,LS;Mann,JJ
通讯作者：
Mann,JJ