MicroRNA-34a targets notch1 and inhibits cell proliferation in glioblastoma multiforme
MicroRNA-34a targets notch1 and inhibits cell proliferation in glioblastoma multiforme
复制标题
MicroRNA-34a 靶向 notch1 并抑制多形性胶质母细胞瘤细胞增殖
DOI：
10.4161/cbt.12.6.16300
复制
发表时间：
2011-09-15
影响因子：
3.6
通讯作者：
Li, Xiao-Rong
中科院分区：
文献类型：
--
作者：
Li, Wen-Bo;Ma, Min-Wang;Li, Xiao-Rong
microRNA (miRNA) 的异常表达与癌症的发生和进展有关。在这项研究中，我们发现与正常脑组织相比，多形性胶质母细胞瘤（GBM）标本中的 microRNA-34a（miR-34a）显着下调。生长曲线和集落形成测定表明，miR-34a 抑制 U373MG 和 SHG44 胶质母细胞瘤细胞的增殖。 miR-34a的过表达可以诱导胶质母细胞瘤细胞凋亡。此外，我们还确定 notch1 是 miR-34a 的直接靶基因。在体外和体内，notch1 的敲低显示出与 miR-34a 过表达相似的细胞功能。总的来说，我们的研究结果表明 miR-34a 在 GBM 细胞中下调，并通过靶向 notch1 抑制 GBM 生长。
Aberrant expression of microRNAs (miRNAs) has been implicated in cancer initiation and progression. In this study, we found that microRNA-34a (miR-34a) is significantly downregulated in glioblastoma multiforme (GBM) specimens compared with normal brain tissues. Growth curve and colony formation assays revealed that miR-34a suppresses proliferation of U373MG and SHG44 glioblastoma cells. Overexpression of miR-34a could induce apoptosis of glioblastoma cells. Also, we identified notch1 as a direct target gene of miR-34a. Knockdown of notch1 showed similar cellular functions as overexpression of miR-34a both in vitro and in vivo. Collectively, our findings show that miR-34a is downregulated in GBM cells and inhibits GBM growth by targeting notch1.