Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells.
Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells.
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DOI：
10.1016/j.isci.2018.02.001
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发表时间：
2018-03-23
期刊：
影响因子：
5.8
通讯作者：
Hansel C
中科院分区：
文献类型：
--
作者：
Ohtsuki G;Hansel C
神经元储存信息并参与记忆痕迹，这是突触重量和膜兴奋性的经验依赖性变化的结果。在这里，我们研究兴奋性突触后电位（EPSP）的幅度和神经元兴奋性的可塑性，这两个机制。我们分析了小脑浦肯野细胞的体细胞-树突状细胞双补丁记录，同时诱导内在的，SK 2通道依赖的可塑性或阻断SK通道浴应用apamin。这两种操作都增加了EPSP训练过程中EPSP振幅的积累，并增加了EPSP诱发的棘波的数量，从而深入了解了EPSP振幅对单个棘波和棘波爆发的机制贡献。EPSP振幅对是否发射尖峰有影响，但兴奋性的直接测量（尖峰阈值/AHP）是更好的预测个体尖峰或尖峰爆发是否被发射。我们的研究结果表明，浦肯野细胞尖峰是突触驱动的，但爆发式放电是由SK2通道调制和可塑性门控的。这项研究是基于浦肯野细胞的体-树突双斑记录。树突记录的EPSP幅度预测锋电位放电的发生。突触重量不能预测单个锋电位或锋电位爆发是否发生。SK2通道调制/可塑性有效地控制锋电位输出。神经科学;细胞神经科学;细胞的专门功能。
Neurons store information and participate in memory engrams as a result of experience-dependent changes in synaptic weights and in membrane excitability. Here, we examine excitatory postsynaptic potential (EPSP) amplitude and neuronal excitability in relation to these two mechanisms of plasticity. We analyze somato-dendritic double-patch recordings from cerebellar Purkinje cells while inducing intrinsic, SK2 channel-dependent plasticity or blocking SK channels with bath application of apamin. Both manipulations increase the build-up of EPSP amplitudes during an EPSP train and enhance the number of EPSP-evoked spikes, yielding insights into the mechanistic contribution of EPSP amplitude to single spikes and spike bursts. EPSP amplitude has an impact on whether spikes are fired or not, but direct measures of excitability (spike threshold/AHP) are better predictors of whether individual spikes or spike bursts are fired. Our findings show that Purkinje cell spiking is synaptically driven but that burst firing is gated by SK2 channel modulation and plasticity. The study is based on somato-dendritic double-patch recordings from Purkinje cells Dendritically recorded EPSP amplitudes predict the occurrence of spike firing Synaptic weight does not predict whether individual spikes or spike bursts occur SK2 channel modulation/plasticity efficiently controls the spike output Neuroscience; Cellular Neuroscience; Specialized Functions of Cells
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