Utilizing "Omic" Technologies to Identify and Prioritize Novel Sources of Resistance to the Oomycete Pathogen Phytophthora infestans in Potato Germplasm Collections.
Utilizing "Omic" Technologies to Identify and Prioritize Novel Sources of Resistance to the Oomycete Pathogen Phytophthora infestans in Potato Germplasm Collections.
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DOI：
10.3389/fpls.2016.00672
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发表时间：
2016
影响因子：
5.6
通讯作者：
Hein I
中科院分区：
文献类型：
--
作者：
Van Weymers PS;Baker K;Chen X;Harrower B;Cooke DE;Gilroy EM;Birch PR;Thilliez GJ;Lees AK;Lynott JS;Armstrong MR;McKenzie G;Bryan GJ;Hein I
世界范围内对马铃薯生产的最大威胁是晚疫病，由卵菌病原体致病疫霉（Phytophthora infestans）引起。用属于基因型13-A2的致病疫霉分离物筛选来自英联邦马铃薯保藏中心（CPC）的126份野生二倍体茄属材料，鉴定了种S. Bulbocastanum、S. capsicibaccatum、辣椒S. microdontum，S. mochiquense、S. okadae，S. pinnatisectum、S. polyadenium，S. tarijense和S.疣状的利用基因组学、等位基因挖掘和诊断RenSeq（dRenSeq）来研究S.冈田的加入。dRenSeq在抗性S. okadae种质7129、7625、3762和大量的20个抗性后代在严格作图条件下证实了全长Rpi-vnt 1.1的存在，并证实了种质7129和7625中的等位基因挖掘结果以及瞬时表达测定中的Avr-vnt 1识别。与此相反，敏感的S.与功能性Rpi-vnt 1.1基因相比，okadae登录号3761和大量20个易感后代在5′端缺乏序列同源性。对S.具有广谱毒力的致病疫霉分离物的冈田种加入证明，尽管S.冈田系种质7129、7625和7629含有功能性Rpi-vnt 1.1，它们还携带一个新的抗性基因。我们提供的证据表明，现有的种质资源是新抗性的重要来源，而基于dRenSeq的基因组学和效应子组学等“组学”技术是快速探索这些种质资源多样性的有效工具。
The greatest threat to potato production world-wide is late blight, caused by the oomycete pathogen Phytophthora infestans. A screen of 126 wild diploid Solanum accessions from the Commonwealth Potato Collection (CPC) with P. infestans isolates belonging to the genotype 13-A2 identified resistances in the species S. bulbocastanum, S. capsicibaccatum, S. microdontum, S. mochiquense, S. okadae, S. pinnatisectum, S. polyadenium, S. tarijense, and S. verrucosum. Effector-omics, allele mining, and diagnostic RenSeq (dRenSeq) were utilized to investigate the nature of resistances in S. okadae accessions. dRenSeq in resistant S. okadae accessions 7129, 7625, 3762, and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 under stringent mapping conditions and corroborated allele mining results in the accessions 7129 and 7625 as well as Avr-vnt1 recognition in transient expression assays. In contrast, susceptible S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5′ end compared to the functional Rpi-vnt1.1 gene. Further evaluation of S. okadae accessions with P. infestans isolates that have a broad spectrum of virulence demonstrated that, although S. okadae accessions 7129, 7625, and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. We provide evidence that existing germplasm collections are important sources of novel resistances and that “omic” technologies such as dRenSeq-based genomics and effector-omics are efficacious tools to rapidly explore the diversity within these collections.