Investigating antibody interactions with a polar liquid using terahertz pulsed spectroscopy.
Investigating antibody interactions with a polar liquid using terahertz pulsed spectroscopy.
复制标题
DOI：
10.1016/j.bpj.2010.11.020
复制
发表时间：
2011-01
影响因子：
3.4
通讯作者：
Yiwen Sun;Yuanting Zhang;E. Pickwell‐MacPherson
中科院分区：
文献类型：
--
作者：
Yiwen Sun;Yuanting Zhang;E. Pickwell‐MacPherson
本文利用太赫兹光谱研究了过氧化物酶亲和纯化羊抗猫免疫球蛋白G和荧光素亲和纯化羊抗猫免疫球蛋白G与极性液体相互作用时的介电性质。蛋白质浓度的影响，以及作为共溶剂的甘油的存在下，通过估计的有效水化壳半径的蛋白质在溶液中。在本研究中的介电谱在0.1-1.3 THz的频率范围内测量，并且发现介电性质取决于氢键抗体网络中的电荷类型。我们的研究结果表明，极性液体的太赫兹介电性能的强烈影响的存在下的抗体，并建议介电谱是特别强大的蛋白质的结构和构象特性的研究。因此，太赫兹光谱是一种非常灵敏的研究生物系统结构特征的方法。
In this article, we use terahertz spectroscopy to study the dielectric properties of the peroxidase-conjugated affinity purified goat anti-cat immunoglobulin G and the fluorescein-conjugated affinity purified goat anti-cat immunoglobulin G when they interact with polar liquids. The influence of protein concentration, as well as presence of glycerol as a cosolvent, is determined by estimation of the effective hydration shell radius of the protein in solution. The dielectric spectra in this study are measured over the frequency range 0.1–1.3 THz and it is found that the dielectric properties are dependent on the type of the charges in the hydrogen-bonded antibodies' networks. Our results indicate that the terahertz dielectric properties of polar liquids are strongly affected by the presence of the antibody and suggest that the dielectric spectrum is particularly powerful in the study of structural and conformational properties of proteins. Therefore, terahertz spectroscopy is a very sensitive approach to investigate structural features of biological systems.