Somatic cell genetic analysis of human cell surface antigens: chromosomal assignments and regulation of expression in rodent-human hybrid cells.
Somatic cell genetic analysis of human cell surface antigens: chromosomal assignments and regulation of expression in rodent-human hybrid cells.
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人类细胞表面抗原的体细胞遗传分析：啮齿动物-人类杂交细胞中的染色体分配和表达调节。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
L. Old
中科院分区：
文献类型：
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作者：
W. Rettig;N. Dracopoli;T. A. Goetzger;B. Spengler;J. L. Biedler;H. Oettgen;L. Old
在一组减少的啮齿动物-人体细胞杂交克隆中研究了13种新定义的由单克隆抗体鉴定的人细胞表面抗原的表达。对于每种抗原系统，抗体反应性的分离与特定人染色体的分离一致，允许确定抗原表达的13个基因位点的染色体分配。抗原可以根据它们在杂交细胞中的控制模式分为四组。(i)单个人染色体的存在对于抗原表达是必要且足够的; L230（分配至染色体2）、AJ 425、K15（染色体3）、SR 84（染色体5）、JF 23、Q14（染色体11）、SV 13（染色体15）和F10（染色体19）。(ii)AJ 2（10号染色体）和J143（17号染色体）;两种抗原由不同的人类染色体编码，但在AJ 2 +/J143+人类细胞表面作为分子复合物结合。(iii)F8（19号染色体）;抗原表达依赖于杂交细胞的生长特征：基质贴壁细胞为F8+，而悬浮生长的细胞为F8-。(iv)AO 122和F23（15号染色体）;抗原表达受啮齿动物融合伴侣的允许/诱导与非允许/非诱导性质控制。来自抗原阳性和抗原阴性人细胞的杂交体可以表达AO 122和F23，但仅当特定的啮齿动物细胞类型用于杂交时：N4 TG-1神经母细胞瘤和L细胞，而不是RAG肾癌细胞，允许AO 122表达，而RAG和L细胞，而不是N4 TG-1细胞，允许F23表达。定义人类细胞表面分子的单克隆抗体的快速扩展列表提供了一系列标记物来探测体细胞中抗原多样性的遗传调节。
The expression of 13 newly defined human cell surface antigens identified by monoclonal antibodies was studied in a panel of reduced rodent-human somatic cell hybrid clones. For each antigenic system the segregation of antibody reactivity was concordant with the segregation of a specific human chromosome, permitting the chromosomal assignment of 13 gene loci determining antigen expression. The antigens can be placed in four groups on the basis of their patterns of control in the hybrid cells. (i) Presence of a single human chromosome is necessary and sufficient for antigen expression; L230 (assigned to chromosome 2), AJ425, K15 (chromosome 3), SR84 (chromosome 5), JF23, Q14 (chromosome 11), SV13 (chromosome 15), and F10 (chromosome 19). (ii) AJ2 (chromosome 10) and J143 (chromosome 17); two antigens coded for by separate human chromosomes but associated as a molecular complex on the surface of AJ2+/J143+ human cells. (iii) F8 (chromosome 19); antigen expression dependent on the growth characteristics of hybrid cells: substrate-adherent cells are F8+, whereas cells growing in suspension are F8-. (iv) AO122 and F23 (chromosome 15); antigen expression controlled by the permissive/inducing vs. nonpermissive/noninducing nature of the rodent fusion partner. Hybrids derived from both antigen-positive and antigen-negative human cells can express AO122 and F23 but only when specific rodent cell types are used for hybridization: N4TG-1 neuroblastoma and L cells, but not RAG renal carcinoma cells, permit AO122 expression, whereas RAG and L cells, but not N4TG-1 cells, permit F23 expression. The rapidly expanding list of monoclonal antibodies defining human cell surface molecules provides a range of markers to probe the genetic regulation of antigen diversity in somatic cells.