Expression of cyclooxygenase-2 in urinary bladder in rats with cyclophosphamide-induced cystitis.
Expression of cyclooxygenase-2 in urinary bladder in rats with cyclophosphamide-induced cystitis.
复制标题
环磷酰胺诱导的膀胱炎大鼠膀胱中环氧合酶-2的表达。
DOI：
10.1152/ajpregu.00305.2007
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Vizzard,MargaretA
中科院分区：
文献类型：
--
作者：
Klinger,MaryBeth;Dattilio,Abbey;Vizzard,MargaretA
这些研究检测了环磷酰胺（CYP）诱导的急性（4小时）、中期（48小时）或慢性（10天）膀胱炎大鼠尿路上皮、尿路上皮下间隙和逼尿肌中环氧合酶-2 （COX-2）的表达。Western blot和免疫组化检测COX-2的表达。在膀胱全贴装制剂中，cypp诱导膀胱炎后，上皮下神经丛神经纤维和炎性细胞浸润均表达COX-2。COX-2在尿路上皮+尿路上皮下和逼尿肌中的表达在急性、中期和慢性（10 d）膀胱炎中均显著升高（P≤0.01），但在尿路上皮+尿路上皮下的表达明显升高（P≤0.01）。尿路上皮+上皮下免疫染色显示的COX-2表达上调与Western blot分析结果相似，也显示COX-2炎症细胞（CD86+）和上皮下神经丛的神经纤维（PGP+）浸润。虽然逼尿肌平滑肌中COX-2表达显著（P≤0.01）升高，但免疫组化未见逼尿肌中COX-2免疫反应性（IR）的明显变化，但整个逼尿肌均可见COX-2炎性浸润。COX-2-IR神经纤维在急性或慢性cyp诱导的膀胱炎时表现出密度增加。COX-2-IR巨噬细胞（CD86+）在急性和慢性cyp诱导的膀胱炎患者的膀胱中均存在。这些研究证明了COX-2抑制剂可能作用于膀胱中的细胞靶点。
These studies examined the expression of cyclooxygenase-2 (COX-2) expression in the urothelium and suburothelial space and detrusor from rats treated with cyclophosphamide (CYP) to induce acute (4 h), intermediate (48 h), or chronic (10-day) cystitis. Western blot analysis and immunohistochemistry were used to demonstrate COX-2 expression. In whole mount preparations of urinary bladder, nerve fibers in the suburothelial plexus, and inflammatory cell infiltrates were characterized for COX-2 expression after CYP-induced cystitis. COX-2 expression significantly (P≤ 0.01) increased in the urothelium + suburothelium and detrusor smooth muscle with acute, intermediate, and chronic (10-day) CYP-induced cystitis, but expression in urothelium + suburothelium was significantly greater. CYP-induced upregulation of COX-2 showed by immunostaining in the urothelium + suburothelium was similar to that observed with Western blot analysis and also demonstrated COX-2 inflammatory cell infiltrates (CD86+) and nerve fibers (PGP+) in the suburothelial plexus. Although COX-2 expression was significantly (P≤ 0.01) increased in detrusor smooth muscle, immunohistochemistry failed to demonstrate an obvious change in COX-2-immunoreactivity (IR) in detrusor muscle, but COX-2 inflammatory infiltrates were present throughout the detrusor. COX-2-IR nerve fibers exhibited increased density in the suburothelial plexus with acute or chronic CYP-induced cystitis. COX-2-IR macrophages (CD86+) were present throughout the urinary bladder with acute and chronic CYP-induced cystitis. These studies demonstrate cellular targets in the urinary bladder where COX-2 inhibitors may act.