Molecular characterization and susceptibility of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from hospitals and the community in Vladivostok, Russia
Molecular characterization and susceptibility of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from hospitals and the community in Vladivostok, Russia
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DOI：
10.1111/j.1469-0691.2009.02891.x
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发表时间：
2010-06-01
影响因子：
14.2
通讯作者：
Suzuki, H.
中科院分区：
文献类型：
--
作者：
Baranovich, T.;Zaraket, H.;Suzuki, H.
在2006年8月至2007年4月的8个月期间进行了一项前瞻性研究，以描述金黄色葡萄球菌相关感染的流行病学。此外，从俄罗斯符拉迪沃斯托克的医院和社区分离的金黄色葡萄球菌进行了分子特征、药物敏感性和耐药性决定因素的鉴定。在符合本研究条件的63株金黄色葡萄球菌中，有48%(n=30)对甲氧西林耐药。医院获得性菌株占所有耐甲氧西林金黄色葡萄球菌(MRSA)的93%(28/30)。主要的MRSA克隆(序列类型(ST)239，葡萄球菌盒式染色体(SCCmec)III型，潘顿-瓦伦丁杀白素(PVL)阴性，以及两个相关的葡萄球菌蛋白A基因(SpA)类型(3型和351型))占所有MRSA分离株的90%。该克隆对多药耐药，41%的分离株对利福平耐药。社区获得的MRSA分离株(n=2)分为ST30、SCCmecIV、SpA19型和PVL阳性，以及新SpA826型和SCCmecIV，而PVL阴性。在甲氧西林敏感的金黄色葡萄球菌(MSSA)中检出8种不同的STS，其中55%为PVL阳性。一个MSSA克隆，被归类为ST121，SpA273型，PVL阳性，导致致命的社区获得性肺炎感染。在俄罗斯医院主要分离的菌株属于耐多药巴西/匈牙利ST239 MRSA克隆；然而，该克隆具有新的抗生素敏感性。此外，还观察到毒力增强的PVL阳性MSSA菌株的出现，需要继续监测。
A prospective study was conducted during an 8-month period, from August 2006 to April 2007, to describe the epidemiology of Staphylococcus aureus-associated infections. In addition, the molecular characteristics, antimicrobial susceptibilities and antibiotic resistance determinants were identified in S. aureus isolates from hospitals and the community in Vladivostok, Russia. Among the 63 S. aureus isolates eligible for this study, methicillin resistance was observed in 48% (n = 30). Hospital-acquired strains accounted for 93% (28/30) of all methicillin-resistant S. aureus (MRSA) isolates. The major MRSA clone (sequence type (ST) 239, staphylococcal cassette chromosome mec (SCCmec) type III, Panton-Valentine leukocidin (PVL)-negative, with two related staphylococcal protein A gene (spa) types (types 3 and 351)) represented 90% of all of the MRSA isolates. This clone was multidrug-resistant, and 41% of isolates showed resistance to rifampicin. Community-acquired MRSA isolates (n = 2) were categorized as ST30, SCCmecIV, spa type 19, and PVL-positive, and as ST8, SCCmecIV, of a novel spa type 826, and PVL-negative. Eight different STs were detected among methicillin-susceptible S. aureus (MSSA) isolates, of which 55% were PVL-positive. One MSSA clone, which was categorized as ST121, spa type 273, and PVL-positive, caused fatal community-acquired pneumonia infections. The strains predominantly isolated in hospitals in Russia belonged to the multidrug-resistant Brazilian/Hungarian ST239 MRSA clone; however, this clone has new antibiotic susceptibilities. Additionally, the emergence of PVL-positive MSSA strains with enhanced virulence was observed, warranting continued surveillance.