Light-Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles
Light-Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles
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DOI：
10.1002/cbic.201900774
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发表时间：
2020-04-07
期刊：
影响因子：
3.2
通讯作者：
Vidakovic-Koch, Tanja
中科院分区：
文献类型：
--
作者：
Kleineberg, Christin;Woelfer, Christian;Vidakovic-Koch, Tanja
结合三磷酸腺苷合成酶和细菌视紫红质的光驱动三磷酸腺苷再生系统已被提出作为合成生物学领域的一种能源供应。在人工创建的反应室(如原始细胞)中进行生化反应需要能量，这些反应室通常基于脂膜或聚合物膜。膜蛋白在不同杂化膜中的插入是微妙的，需要研究将这些系统与脂质体进行比较。本文详细研究了接枝聚合物PDMS-g-PEO和两嵌段共聚物PBD-PEO在不同杂化隔室中的膜蛋白功能。脂类/聚合物杂化囊泡的活性可达90%以上，具有良好的生物相容性。聚合物/聚合物杂化化合物的长期稳定性显著提高(42天后仍有80%的活性)。
Light-driven ATP regeneration systems combining ATP synthase and bacteriorhodopsin have been proposed as an energy supply in the field of synthetic biology. Energy is required to power biochemical reactions within artificially created reaction compartments like protocells, which are typically based on either lipid or polymer membranes. The insertion of membrane proteins into different hybrid membranes is delicate, and studies comparing these systems with liposomes are needed. Here we present a detailed study of membrane protein functionality in different hybrid compartments made of graft polymer PDMS-g-PEO and diblock copolymer PBd-PEO. Activity of more than 90 % in lipid/polymer-based hybrid vesicles could prove an excellent biocompatibility. A significant enhancement of long-term stability (80 % remaining activity after 42 days) could be demonstrated in polymer/polymer-based hybrids.