Influence of redox mediators and metal ions on synthetic acid dye decolourization by crude laccase from Trametes hirsuta
Influence of redox mediators and metal ions on synthetic acid dye decolourization by crude laccase from Trametes hirsuta
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DOI：
10.1016/j.chemosphere.2004.09.033
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发表时间：
2005-01-01
期刊：
影响因子：
8.8
通讯作者：
Gübitz, GM
中科院分区：
文献类型：
--
作者：
Couto, SR;Sanromán, M;Gübitz, GM
本文研究了氧化还原介质对毛毡菌漆酶对合成酸性染料（Sella Solid Red和Luganil Green）脱色的影响。2,2′-氮基-双（3-乙基苯并噻唑-6-磺酸）（ABTS）、1-羟基苯并三唑（HBT）和瑞马唑亮蓝R （RBBR）三种氧化还原介质的活性均高于未添加介质的活性，表明漆酶/介质体系（LMS）在酸性染料脱色中的适用性。HBT是迄今为止最有效的调节剂。Sella Solid Red在10分钟内脱色率为88%，Luganil Green在20分钟内脱色率为49%。另一方面，评价了漆酶对纺织废水中常见的几种金属离子的稳定性。漆酶对除Zn+2外的所有金属离子在1 mM浓度下稳定7d。CrO4-2, Cd+2, Cr2O7-2, Fe+2, Cu+2，尤其是Hg+2。当浓度增加到10mM时，漆酶对所有被测金属的稳定性下降，特别是对Fe+2。此外，还研究了金属离子对脱色过程的影响。发现Hg+2对染料脱色过程有抑制作用，因为脱色所必需的HBT的存在。(C) 2004 Elsevier Ltd.版权所有。
In this paper, the effect of redox mediators on synthetic acid dye decolourization (Sella Solid Red and Luganil Green) by laccase from Trametes hirsuta cultures has been investigated. All the redox mediators tested, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1-hydroxybenzotriazole (HBT) and Remazol Brilliant Blue R (RBBR), led to higher activities than those obtained without mediators addition showing the suitability of the laccase/mediator system (LMS) in the decolourization of acid dyes. HBT was by far the most effective mediator. shoving a decolourization percentage of 88% in 10min for Sella Solid Red and of 49% in 20min for Luganil Green.On the other hand, the stability of laccase against several metal ions, normally found in textile wastewater, was assessed. Laccase was stable at a concentration of 1 mM for 7d against all the metal ions tested except for Zn+2. CrO4-2, Cd+2, Cr2O7-2, Fe+2, Cu+2 and especially Hg+2. When the concentration was increased to 10mM laccase stability decreased against all the metals assayed, in particular against Fe+2. In addition, the effect of metal ions on the decolourization process was also studied. It was found that Hg+2 inhibited the dye decolourization process, being the presence, of HBT absolutely required for dye decolourization. (C) 2004 Elsevier Ltd. All rights reserved.