Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point.
Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point.
复制标题
使用组织培养和 MTT 终点对临床胃肠癌进行化学敏感性测试。
作者：
T. Furukawa;T. Kubota;M. Watanabe;S. Kase;T. Takahara;H. Yamaguchi;T. Takeuchi;T. Teramoto;K. Ishibiki;M. Kitajima
采用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基- 2h溴化四唑铵（MTT）终点的三维组织培养方法对100例临床胃肠道肿瘤进行了化疗敏感性测试，并将结果与MTT终点的细胞悬浮培养结果进行了比较。在100个手术标本中，91个可以用组织培养法评估，66个可以用细胞悬浮法评估。当实验结果与历史上临床化疗反应的频率进行比较时，组织培养实验的结果比细胞悬浮实验的结果显示出更密切的相关性。因此，与细胞悬液试验相比，组织培养试验似乎具有更高的评估率，并且与胃肠道癌症的临床化疗敏感性更密切相关。
Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.