Flavonoids and Phenolic Compounds from Rosmarinus officinalis
Flavonoids and Phenolic Compounds from Rosmarinus officinalis
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DOI：
10.1021/jf100332w
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发表时间：
2010-05-12
影响因子：
6.1
通讯作者：
Ho, Chi-Tang
中科院分区：
文献类型：
--
作者：
Bai, Naisheng;He, Kan;Ho, Chi-Tang
本文报道了一个新的黄酮类化合物6“-O-（E）-feruloylhomoplantaginin（1）和14个已知化合物6”-O-（E）-feruloylnepitrin（2），6“-O-（E）-p-coumaroylnepitrin（3），6-甲氧基毛地黄黄酮7-葡萄糖苷（4），毛地黄黄酮葡萄糖苷酸（5），毛地黄黄酮3 '-O-（3“-O-乙酰基）-β-D-葡糖苷酸（6）、山奈酚（7）、毛地黄黄酮（8）、芫花素（9）和拉达因（10），以及1-O-阿魏酰-β-O-吡喃葡萄糖（11）、1-O-从迷迭香（Rosmarinus officinalis）叶中分离得到4-羟基苯甲酰基-β-D-吡喃葡萄糖（12）、迷迭香酸（13）、鼠尾草酸（14）和鼠尾草酚（15）。基于HRMS支持的NMR光谱方法确定结构。测试所有分离的化合物在人癌细胞系（HepG 2、科洛205和HL-60）中的细胞毒性和在脂多糖（LPS）处理的RAW 264.7巨噬细胞中的抗炎活性。其中，化合物14和15在抑制巨噬细胞中亚硝酸盐产生方面具有适度活性，其次是化合物8和5。化合物14和15作为HL-60细胞中的抗增殖剂更有效，其IC 50值为1.7和5.5 μ M，其次是化合物8和7，其IC 50分别为39.6和82.0 μ M。此外，化合物14和15对科洛205细胞显示出有效的抗增殖作用，其IC 50值分别为32.8和29.9 μ M。
A new flavonoid, 6 ''-O-(E)-feruloylhomoplantaginin (1), and 14 known compounds, 6 ''-O-(E)-feruloylnepitrin (2), 6 ''-O-(E)-p-coumaroylnepitrin (3), 6-rnethoxyluteolin 7-glucopyranoside (4), luteolin glucuronide (5), luteolin 3'-O-(3 ''-O-acetyl)-beta-D-glucuronide (6), kaempferol (7), luteolin (8), genkwanin (9), and ladanein (10), together with 1-O-feruloyl-beta-O-glucopyranose (11), 1-O-(4-hydroxybenzoy1)-beta-D-glucopyranose (12), rosmarinic acid (13), camosic acid (14), and carnosol (15), were isolated from the leaves of Rosmarinus officinalis. The structures were established on the basis of NMR spectroscopic methods supported by HRMS. All isolated compounds were tested for cytotoxicity in human cancer cell lines (HepG2, COLO 205, and HL-60) and for anti-inflammatory activities in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. Among them, compounds 14 and 15 were modestly active in the inhibition of nitrite production in macrophages, followed by compounds 8 and 5. Compounds 14 and 15 were more effective as an antiproliferative agent in HL-60 cells with IC50 values of 1.7 and 5.5 mu M, followed by compounds 8 and 7 with IC50 of 39.6 and 82.0 mu M, respectively. In addition, compounds 14 and 15 showed potent antiproliferative effects on COLO 205 cells with IC50 values of 32.8 and 29.9 mu M, respectively.