Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells
Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells
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DOI：
10.1063/5.0023744
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发表时间：
2020-10-28
影响因子：
3.2
通讯作者：
Clark, Matt
中科院分区：
文献类型：
--
作者：
Perez-Cota, Fernando;Fuentes-Dominguez, Rafael;Clark, Matt
表征生物细胞的弹性正在成为深入了解细胞生物学的一种新方法。细胞力学与细胞行为的大多数方面有关，在研究和医学中的应用非常广泛。目前的方法往往是有限的，因为它们需要身体接触或缺乏分辨率。从可用于表征弹性的方法中，那些依赖于高频超声（声子）的方法是最有前途的，因为它们提供了无标记，高（甚至超光学）分辨率和与传统光学显微镜的兼容性。在这方面的贡献，我们回顾了皮秒超声波细胞成像和表征，特别是布里渊散射为基础的方法，提供了一个意见所面临的技术挑战的最新状态。挑战分为生物相容性，采集速度，分辨率和数据解释，并详细讨论了沿着新的结果。
Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad. Current methods are often limited since they require physical contact or lack resolution. From the methods available for the characterization of elasticity, those relying on high frequency ultrasound (phonons) are the most promising because they offer label-free, high (even super-optical) resolution and compatibility with conventional optical microscopes. In this Perspective contribution, we review the state of the art of picosecond ultrasonics for cell imaging and characterization, particularly for Brillouin scattering-based methods, offering an opinion for the challenges faced by the technology. The challenges are separated into biocompatibility, acquisition speed, resolution, and data interpretation and are discussed in detail along with new results.