Hypoxic coordinate regulation of mitochondrial enzymes in mammalian cells.
Hypoxic coordinate regulation of mitochondrial enzymes in mammalian cells.
复制标题
哺乳动物细胞中线粒体酶的缺氧协调调节。
DOI：
10.1126/science.6320368
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Clayton,DA
中科院分区：
文献类型：
--
作者：
Murphy,BJ;Robin,ED;Tapper,DP;Wong,RJ;Clayton,DA
在两种哺乳动物细胞中研究了低氧暴露对多种线粒体酶的影响和对细胞线粒体基因组含量的影响。低氧对六种酶活性均有不同程度的抑制作用。在常氧再暴露期间，三种标志酶的抑郁和恢复动力学在统计学上是相似的。尽管酶活性呈全局性和对称性下降，但线粒体DNA保持不变。这表明，要么是所有线粒体的线粒体酶对称性丧失，要么是线粒体亚群的酶完全丧失，但保留了完整的线粒体基因组。
The effect of hypoxic exposure on various mitochondrial enzymes and on cell mitochondrial genomic content was studied in two types of mammalian cells. Hypoxia depressed the activity of six enzymes to the same degree. The kinetics of depression and of recovery during reexposure to normoxia were statistically similar for three marker enzymes. Despite the global and symmetrical decrease in enzyme activities, mitochondrial DNA remained constant. This suggests either symmetrical loss of mitochondrial enzymes from all mitochondria or complete loss of enzymes from a subpopulation of mitochondria with retention of an intact mitochondrial genome.