Thyrotropin-releasing hormone and gonadotropin-releasing hormone-associated peptide modulation of [Ca2+]i in human lactotrophs
Thyrotropin-releasing hormone and gonadotropin-releasing hormone-associated peptide modulation of [Ca2+]i in human lactotrophs
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促甲状腺激素释放激素和促性腺激素释放激素相关肽对人泌乳素细胞中 [Ca2]i 的调节
DOI：
10.1016/0303-7207(93)90030-n
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发表时间：
1993
影响因子：
4.1
通讯作者：
J. Guerin
中科院分区：
文献类型：
--
作者：
L. Dufy;L. Bresson;P. Vacher;M. Odessa;F. Galli;J. Rivel;J. Guerin
研究了促甲状腺素释放激素 (TRH) 和促性腺激素释放激素相关肽 (GAP) 对从催乳素 (PRL) 分泌肿瘤培养的人垂体细胞中的分泌和细胞内游离 Ca2+ 浓度 ([Ca2+]i) 的影响。在 30 分钟的孵育期内测量分泌量，我们在单个细胞中使用显微分光荧光法，并以 indo-1 作为荧光探针。在这 5 个细胞群中，有 5 个细胞群中的 TRH (10−8M) 显着增加了 PRL 的释放，而在另一种培养物中，[Ca2+]i 的增加没有发现 PRL 分泌的显着增加。在其中 3 个细胞群中研究了 GAP (10−7M) 的影响，减少了 20%。在这些培养物中，GAP 分别抑制了 59%、46% 和 94% 的 PRL 基础分泌率。百日咳毒素 (PT) 预处理阻断了 GAP 的抑制作用，这表明在其他两种培养物中，GAP 没有显着改变 PRL 分泌或单个细胞的 [Ca2+]i。观察表明，GAP 可能在控制人体 PRL 分泌中发挥作用。
The effect of thyrotropin-releasing hormone (TRH) and gonadotropin-releasing hormone-associated peptide (GAP) was studied on both secretion and intracellular free Ca2+concentrations ([Ca2+]iin human pituitary cells cultured from prolactin (PRL)-secreting tumors. Secretion was measured during a 30-min incubation period and we used a microspectrofluorimetric method in individual cells and indo-1 as the fluorescent probe. TRH (10−8M) significantly increased PRL release in five out of the six cell populations. In these five cases, more than 68% of individual cells responded to TRH by an increase in [Ca2+]i. No significant increase in PRL secretion was found in another culture in which TRH increased [Ca2+]iin only 37% of the cells. The effect of GAP (10−7M) was studied in five cell populations. In three of them, a decrease of 20% to 51% of the PRL basal secretory rate was observed under GAP. GAP inhibited [Ca2+]iin respectively 59%, 46% and 94% of the cells from these cultures. The inhibitory effect of GAP was blocked by a pertussis toxin (PT) pretreatment which demonstrates the involvement of a PT-sensitive G-protein in GAP action. In two other cultures, GAP did not significantly alter PRL secretion or individual cell [Ca2+]i. These observations suggest that GAP might play a role in the control of PRL secretion in the human.
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DOI：
10.1016/s0021-9258(17)42556-7
发表时间：
1984-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Albert;A. Tashjian
通讯作者：
P. Albert;A. Tashjian
促性腺激素释放激素相关肽在体内发挥催乳素抑制和弱促性腺激素释放活性。
影响因子：
4.8
作者：
Yu,WH;Seeburg,PH;Nikolics,K;McCann,SM
通讯作者：
McCann,SM
促甲状腺激素释放激素 (TRH) 刺激 GH3 细胞中细胞质游离钙的双相升高。
影响因子：
4.8
作者：
Gershengorn,MC;Thaw,C
通讯作者：
Thaw,C
影响因子：
18.2
作者：
Patel,YC;Srikant,CB
通讯作者：
Srikant,CB
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Holl,RW;Thorner,MO;Mandell,GL;Sullivan,JA;Sinha,YN;Leong,DA
通讯作者：
Leong,DA