Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos.
Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos.
复制标题
Xwnt-3A 和 Xwnt-1 在非洲爪蟾胚胎神经组织中的重叠表达。
DOI：
10.1006/dbio.1993.1005
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
Moon,RT
中科院分区：
文献类型：
--
作者：
Wolda,SL;Moody,CJ;Moon,RT
Xwnt-3A是非洲爪蟾Wnt基因家族的成员，该家族是一类分泌的富含半胱氨酸的蛋白质，在早期发育期间参与细胞间信号传导。本文描述了Xwnt-3A的全长切割序列，以及通过全基因组原位杂交分析确定的Xwnt基因的空间表达模式。Xwnt-3A与Wnt-3（87%）和Wnt-3A（85%）的氨基酸同源性很高，但其空间表达模式与Wnt-3A最相似。Xwnt-3A最早在神经胚发育阶段被检测到，仅沿着发育中的脑和神经管的背中线和沿着耳泡的背表面表达。虽然Xwnt-1的表达与Xwnt-3A的表达广泛重叠，但Xwnt-1仅沿着中脑/后脑边界表达，并且在耳泡中不存在。Xwnt-3A在神经外胚层中的表达依赖于神经诱导，如用重组外胚层和中胚层组织的实验所确定的。这些结果表明Xwnt-3A可能参与了Xenopus早期发育过程中中枢神经系统的模式化。最后，Xwnt-3A的异位表达诱导胚胎前端次级轴的形成。
Xwnt-3A, is a member of theXenopus Wntgene family, a class of secreted, cysteine-rich proteins implicated in intercellular signaling during early development. Here we describe the full-length ceding sequence ofXwnt-3A, as well as the spatial expression pattern of thisXwntgene as determined by whole-mountin situhybridization analysis. WhileXwnt-3Ashares considerable amino acid identity with bothWnt-3(87%) andWnt-3A(85%), its spatial expression pattern is most like that ofWnt-3A.Xwnt-3A, which is first detected at the neurula stage of development, is expressed exclusively along the dorsal midline of the developing brain and neural tube and along the dorsal surface of the otic vesicle. While the expression ofXwnt-1extensively overlaps that ofXwnt-3A, Xwnt-1is uniquely expressed along the midbrain/hindbrain boundary and is absent from the otic vesicle. The expression ofXwnt-3Ain neural ectoderm is dependent upon neural induction as determined by experiments with recombined ectoderm and mesoderm tissue. These results suggest thatXwnt-3Amay participate in patterning the central nervous system during earlyXenopusdevelopment. Last, the ectopic expression ofXwnt-3Ainduces the formation of a secondary axis at the anterior end of the embryo.