In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1.
In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1.
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DOI：
10.1039/c3cc44986f
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发表时间：
2013-08
影响因子：
4.9
通讯作者：
Dong-Dong Zheng-Dong;D. Pan;Xiao Zha;Yuqing Wu;Chunlai Jiang;Xianghui Yu
中科院分区：
文献类型：
--
作者：
Dong-Dong Zheng-Dong;D. Pan;Xiao Zha;Yuqing Wu;Chunlai Jiang;Xianghui Yu
The recombinant GST fusion protein HPV 16 L1 from E. coli was proved to exist as a monomer rather than a pentamer, providing the possibility of real-time monitoring of the pentamer formation in vitro. Time-dependent kinetic studies of the process were performed for the first time by using static light scattering and western blot analysis, where the essential factors were revealed, offering a new biotechnical approach for virus control and/or the development of anti-viral agents.
