POLYMORPHISM OF BRAIN TUBULIN
POLYMORPHISM OF BRAIN TUBULIN
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DOI：
10.1021/bi00512a006
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
LEE, JC
中科院分区：
文献类型：
--
作者：
GEORGE, HJ;MISRA, L;LEE, JC
对小牛脑微管蛋白进行等电聚焦和胰蛋白酶肽图分析。等电聚焦实验的结果显示了17个良好分辨的蛋白质峰的总数。当蛋白质或两性电解质的浓度改变时，峰的数目和每个峰下的质量分布保持相同。当蛋白质进行2维等电聚焦时，观察到对角线图案，表明观察到的多个峰不是微管蛋白-两性电解质相互作用的表现。通过分离这些单独的亚种并对其进行等电聚焦的进一步研究产生了与原始峰相对应的单峰，而没有产生多峰的初始模式。胰蛋白酶肽图谱显示，在α.亚基共有26个位点。有7个。+-。1点是独特的每个亚种。对于β的亚种也获得了类似的观察结果。亚基，尽管只有2 ±。每个亚种有1个独特的斑点。这些结果表明，微管蛋白亚基可能由多肽的恒定区和可变区在其序列。犬和兔脑微管蛋白获得相同的结果，表明微管蛋白多态性是常见的脑组织。通过聚合-解聚或修改的Weisenberg程序分离的微管蛋白产生相同的结果。这些结果表明，通过两种分离方法提取了相同的微管蛋白亚种。
Calf brain tubulin was subjected to isoelectric focusing and tryptic peptide map analysis. Results from isoelectric focusing experiments showed a total number of 17 well-resolved protein peaks. The number of peaks and the mass distribution under each peak remained the same when the concentration of protein or ampholyte was altered. When the protein was subjected to 2-dimensional isoelectric focusing, a diagonal pattern was observed, indicating that the multiple peaks observed are not a manifestation of tubulin-ampholyte interaction. Further investigation by isolating these individual subspecies and subjecting them to isoelectric focusing yielded single peaks corresponding to the orignal ones without generating the initial pattern of multiple peaks. Tryptic peptide maps showed that among the subspecies of the .alpha. subunit there are 26 spots that are common among them. There are 7 .+-. 1 spots that are unique in each subspecies. Similar observations were obtained for the subspecies of the .beta. subunit although there are only 2 .+-. 1 unique spots in each subspecies. These results suggest that tubulin subunits probably consist of polypeptides with both constant and variable regions in their sequences. Identical results were obtained for canine and rabbit brain tubulin, indicating that tubulin polymorphism is common among brain tissues. Tubulin isolated by either the polymerization-depolymerization or the modified Weisenberg procedures yielded identical results. These results show that the same subspecies of tubulin are extracted by both isolation procedures.