Timing of preweanling maternal effects on development of hypertension in SHR rats.
Timing of preweanling maternal effects on development of hypertension in SHR rats.
复制标题
断奶前母体对 SHR 大鼠高血压发生的影响的时间。
DOI：
10.1016/0031-9384(94)90069-8
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发表时间：
1994
影响因子：
2.9
通讯作者：
Fields-Okotcha,C
中科院分区：
文献类型：
--
作者：
McCarty,R;Fields-Okotcha,C
本研究采用胎鼠交叉饲养的方法，探讨了母鼠断奶前对自发性高血压（SHR）大鼠高血压发生的影响。在出生后1-7天、1-14天、1-21天、8-21天或15-21天，SHR幼崽要么由其自然母亲饲养，要么与正常Wistar-Kyoto （WKY）母亲相互交叉培养。所有SHR幼崽在21日龄断奶，雄鼠每笼饲养2至3只，直到100日龄获得生理指标。在100日龄时，所有大鼠手术准备尾动脉导管，并在手术后的第二天，在大鼠休息和不受干扰的情况下，直接测量平均动脉压（MAP, mmHg）和心率（HR, bpm）。我们的研究结果表明，与WKY母鼠交叉饲养的SHR幼崽断奶时的体重和成年后的map有显著影响。与对照组相比，除15 - 21天组外，交叉培养的仔猪在断奶时明显更重。到100日龄时，各处理组的体重基本一致。杂交饲养1-7天、1-14天、1-21天和8-21天的SHR的基础map与自然母饲养的SHR对照相比显著降低，但15-21天没有。相比之下，任何交叉培养SHR组的基础hr都没有受到影响。这些数据表明，产妇环境对SHRs高血压发展的影响仅限于出生后的前两周。这与断奶前的时间相对应，当老鼠幼崽从它们的母亲那里获得所有的营养。
Cross-fostering of litters was used to determine the timing of preweanling maternal influences on the development of high blood pressure in spontaneously hypertensive (SHR) rats. The SHR litters were either reared by their natural mothers or reciprocally cross-fostered to normotensive Wistar-Kyoto (WKY) mothers for postnatal days 1–7, 1–14, 1–21, 8–21, or 15–21. All SHR litters were weaned at 21 days of age and males were housed in groups of two to three per cage until physiological measures were obtained at 100 days of age. At 100 days of age, all rats were surgically prepared with tail artery catheters and, on the day after surgery, direct measures of mean arterial pressure (MAP, mmHg) and heart rate (HR, bpm) were obtained while rats were resting and undisturbed in their individual home cages. Our findings indicate that cross-fostering SHR pups to WKY foster mothers was attended by significant effects on body weights at weaning and on adult MAPs. Compared to control SHRs, cross-fostered SHRs, with the exception of the 15–21-day group, were significantly heavier at weaning. By 100 days of age, body weights of SHRs were similar across treatment groups. Basal MAPs of SHRs cross-fostered for days 1–7, 1–14, 1–21, and 8–21, but not days 15–21, were reduced significantly compared to SHR controls reared by their natural mothers. In contrast, basal HRs were not affected in any of the cross-fostered SHR groups. These data indicate that maternal environmental influences on the development of high blood pressure in SHRs are limited to the first 2 weeks of postnatal life. This corresponds to the time prior to weaning when rat pups receive all of their nourishment from their mothers.