Orientation Imaging and Reorientation Dynamics of Single Dye Molecules
Orientation Imaging and Reorientation Dynamics of Single Dye Molecules
复制标题
单染料分子的取向成像和重新取向动力学
DOI：
10.1021/jp001373p
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发表时间：
2001
影响因子：
3.3
通讯作者：
L. Goldner
中科院分区：
文献类型：
--
作者：
Kenneth D. Weston;L. Goldner
介绍了获取单分子定向图像和分析单荧光分子定向动力学的新技术。偏振调制与共聚焦显微镜用于测量在环境条件下物理吸附到玻璃上并嵌入到薄的自旋铸造聚合物薄膜中的单个染料分子的吸收偶极子取向和重取向动力学。在所有测试的样品中，对相当一部分染料分子观察到吸收偶极子取向的离散跳跃，并且在所有情况下，首次跳跃时间的分布都可以通过拉伸指数函数来拟合。观察到染料分子的亚群在这些实验的时间尺度（32秒）上是静止的，即使在高激发功率下也会持续存在。数据分析技术的发展量化了单分子的重定向动力学，并介绍了一种在扫描场中快速识别旋转移动分子的新方法。对现实的依赖……
We introduce new techniques for obtaining single-molecule orientation images and analyzing the reorientation dynamics of single fluorescent molecules. Polarization modulation is used with confocal microscopy to measure the absorption dipole orientation and reorientation dynamics of individual dye molecules physisorbed to glass and embedded in thin, spin-cast polymer films under ambient conditions. Discrete jumps in absorption dipole orientation are observed for a significant fraction of dye molecules in all samples tested, and in all cases the distribution of first-jump times can be fitted by a stretched exponential function. A sub-population of dye molecules that is stationary on the time scale of these experiments (32 s) is observed and persists even at high excitation power. Data analysis techniques are developed for quantifying the reorientation dynamics of single molecules, and a novel method for quickly identifying rotationally mobile molecules in a scan field is introduced. A dependence of the reor...