Point-of-care seeding of nitinol stents with blood-derived endothelial cells.
Point-of-care seeding of nitinol stents with blood-derived endothelial cells.
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用血液来源的内皮细胞进行镍钛合金支架的护理点接种。
DOI：
10.1002/jbm.b.33510
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Achneck,HardeanE
中科院分区：
文献类型：
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作者：
Jantzen,AlexandraE;Noviani,Maria;Mills,JamesS;Baker,KatherineM;Lin,Fu-Hsiung;Truskey,GeorgeA;Achneck,HardeanE
基于镍钛合金的血管装置，例如外周支架和颅内支架，受到血栓形成和再狭窄的限制。为了改善这些并发症，我们开发了一种技术，通过在植入前立即将自体血液来源的内皮细胞 (EC) 快速播种 (QuickSeeding) 到改良的自膨胀镍钛合金支架输送系统上来促进血管愈合。在商业镍钛合金支架周围的输送系统护套中激光钻出数千个微孔，以允许输注的细胞悬浮液排出。当悬浮介质通过微孔向外流动时，内皮细胞流经输送系统附着在支架表面。在静态或流动条件下进行 24 小时体外培养后，QuickSeeded ECs 粘附在支架表面并铺展。此外，部署到猪颈动脉的支架上的 QuickSeeded EC 在 48 小时内扩散，使支架支柱内皮化 (n= 4)。与静态条件相比，24 小时后，QuickSeededstent 支柱在离体流动回路中产生显着更多的一氧化氮 (n= 5)。总之，ECsQuickSeededonto 商业镍钛合金支架在植入后几分钟内即可扩散，在体外和体内形成功能层，这提供了概念证明，即具有改进的输送系统的新型 QuickSeeding 方法可用于在护理点接种功能性自体内皮。 © 2015 Wiley periodicals, Inc. J Biomed Mater Res B 部分：Appl Biomater，104B：1658–1665，2016。
Nitinol‐based vascular devices, for example, peripheral and intracranial stents, are limited by thrombosis and restenosis. To ameliorate these complications, we developed a technology to promote vessel healing by rapidly seeding (QuickSeeding) autologous blood‐derived endothelial cells (ECs) onto modified self‐expanding nitinol stent delivery systems immediately before implantation. Several thousand micropores were laser‐drilled into a delivery system sheath surrounding a commercial nitinol stent to allow for exit of an infused cell suspension. As suspension medium flowed outward through the micropores, ECs flowed through the delivery system attaching to the stent surface. TheQuickSeededECs adhered to and spread on the stent surface following 24‐hin vitroculture under static or flow conditions. Further,QuickSeededECs on stents that were deployed into porcine carotid arteries spread to endothelialize stent struts within 48 h (n= 4). TheQuickSeededstent struts produced significantly more nitric oxide inex vivoflow circuits after 24 h, as compared to static conditions (n= 5). In conclusion, ECsQuickSeededonto commercial nitinol stents within minutes of implantation spread to form a functional layerin vitroandin vivo, providing proof of concept that the novelQuickSeedingmethod with modified delivery systems can be used to seed functional autologous endothelium at the point of care. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1658–1665, 2016.