DNA-DEGRADATION IN TERMINALLY DIFFERENTIATING LENS FIBER CELLS FROM CHICK-EMBRYOS
DNA-DEGRADATION IN TERMINALLY DIFFERENTIATING LENS FIBER CELLS FROM CHICK-EMBRYOS
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DOI：
10.1073/pnas.74.12.5579
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
MODAK, SP
中科院分区：
文献类型：
--
作者：
APPLEBY, DW;MODAK, SP
在晶状体纤维细胞的终末分化过程中，细胞核DNA积累了游离的3”-OH末端，并逐渐从细胞核中丢失。在这一过程接近尾声时，细胞核收缩并消失。用非变性琼脂糖/聚丙烯酰胺凝胶电泳检测上皮细胞和纤维细胞发育早期和晚期DNA的大小。离散大小的低分子量DNA仅出现在中央纤维细胞核变性的最后阶段，并在细胞核消失后持续存在。这些低分子量DNA片段表现为单个单元的倍数，与微球菌核酸酶消化上皮细胞核产生的片段相似。在晶态纤维核中，体内双链断裂在核变性过程中影响染色质，这些细胞的DNA被组织成由离散亚基组成的染色质。
During the terminal differentiation of lens fiber cells, nuclear DNA accumulates free 3''-OH ends and is progressively lost from the nucleus. Toward the end of this process, nuclei undergo pycnosis and disappear. The size of the DNA in the epithelia and in early and late stages of fiber cell development was examined by electrophoresis on nondenaturing agarose/polyacrylamide gels. Low MW DNA of discrete sizes appears only at the final stages of nuclear degeneration in central fiber cells and persists after the disappearance of the nuclei. These low MW DNA fragments appear as multiples of a monomeric unit and are similar to the fragments produced by the digestion of epithelial cell nuclei by micrococcal nuclease. In lens fiber nuclei the double-strand breaks in vivo affect the chromatin during nuclear degeneration, and the DNA of these cells is organized into chromatin composed of discrete subunits.