Expression of neurturin, GDNF, and GDNF family-receptor mRNA in the developing and mature mouse
Expression of neurturin, GDNF, and GDNF family-receptor mRNA in the developing and mature mouse
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DOI：
10.1006/exnr.1999.7127
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发表时间：
1999-08-01
影响因子：
5.3
通讯作者：
Johnson, EM
中科院分区：
文献类型：
--
作者：
Golden, JP;DeMaro, JA;Johnson, EM
神经营养因子家族目前有四个成员：神经肽(NRTN)、胶质细胞源性神经营养因子(GDNF)、Persephin和青蒿素。这些蛋白质是几个中枢和外周神经元群体的有效生存因素。这些因子的受体是包括Ret酪氨酸激酶受体和GPI连接的配体结合成分的复合体，称为GDNF家族受体α1-4(GFRα1-4)。我们用原位杂交的方法研究了NRTN、GDNF、Ret、GFRα1和GFRα2在胚胎发育过程中和成年小鼠中的表达。在胚胎发育过程中，GDNF受体在神经系统、泌尿生殖系统、消化系统、呼吸系统以及发育中的皮肤、骨骼、肌肉和内分泌腺中显著表达。在一些区域，表达了不完整的受体复合体，这表明存在其他尚未鉴定的受体组分，或者在反式中形成了受体复合体。NRTN和GDNF仅在胚胎发育过程中的许多三叉神经靶组织中表达，包括鼻黏膜上皮、牙齿和胡须毛囊。NRTN和GDNF在发育中的肢体和泌尿生殖系统也有表达。在胚胎中，GDNF因子和受体在间质/上皮诱导的几个部位表达，包括肾脏、牙齿和颌下腺。这种表达模式与GDNF因子在胚胎发育的诱导过程中发挥作用的可能性以及最近发现的NRTN作为一些副交感神经元发育所必需的营养因子的作用是一致的。在成熟动物中，受体的表达比在胚胎中更有限。在成年小鼠中，NRTN在肠道、前列腺、睾丸和输卵管中表达最显著，GDNF在卵巢中最显著表达。(C)1999年学术出版社。
The GDNF family of neurotrophic factors currently has four members: neurturin (NRTN), glial cell line-derived neurotrophic factor (GDNF), persephin, and artemin. These proteins are potent survival factors for several populations of central and peripheral neurons. The receptors for these factors are complexes that include the Ret tyrosine kinase receptor and a GPI-linked, ligand-binding component called GDNF family receptor alpha 1-4 (GFR alpha 1-4). We have used in situ hybridization to study the mRNA expression of NRTN, GDNF, Ret, GFR alpha 1, and GFR alpha 2 during embryonic development and in the adult mouse. GDNF receptors were prominently expressed during embryonic development in the nervous system, the urogenital system, the digestive system, the respiratory system, and in developing skin, bone, muscle, and endocrine glands. In some regions, incomplete receptor complexes were expressed suggesting that other, as yet unidentified, receptor components exist or that receptor complexes are formed in trans. NRTN and GDNF mere expressed in many trigeminal targets during embryonic development including the nasal epithelium, the teeth, and the whisker follicles. NRTN and GDNF were also expressed in the developing Limbs and urogenital system. In the embryo, GDNF factors and receptors were expressed at several sites of mesenchyme/epithelial induction, including the kidney, tooth, and submandibular gland. This expression pattern is consistent with the possibility that the GDNF factors function in inductive processes during embryonic development and with the recently discovered role of NRTN as a necessary trophic factor for the development of some parasympathetic neurons. In the mature animal, receptor expression was more limited than in the embryo. In the adult mouse, NRTN was most prominently expressed in the gut, prostate testicle, and oviduct; GDNF was most prominently expressed in the ovary. (C) 1999 Academic Press.