Stress-induced galectin-1 influences immune tolerance in the spleen and thymus by modulating CD45 immunoreactive lymphocytes.
Stress-induced galectin-1 influences immune tolerance in the spleen and thymus by modulating CD45 immunoreactive lymphocytes.
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DOI：
10.1007/s12576-016-0478-8
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发表时间：
2017-07
期刊：
影响因子：
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通讯作者：
Kadoya T
中科院分区：
文献类型：
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作者：
Sasaguri K;Yamada K;Narimatsu Y;Oonuki M;Oishi A;Koda K;Kubo KY;Yamamoto T;Kadoya T
Galectin-1(Galectin-1，Gal-1)在正常组织和病理组织中差异表达，调节免疫细胞的动态平衡。束缚应激可增加大鼠血清Gal-1水平。然而，应激诱导血清中Gal-1的功能尚不清楚。我们确定了应激诱导的Gal-1是否在免疫活性器官中积累，以保护其免受生理和/或心理应激的影响。Western blotting结果显示，应激组Gal-1条带强度明显高于对照组。RT-PCR分析表明，束缚应激后Gal-1mRNA水平无明显变化。应激组大鼠脾动脉周围淋巴鞘和胸腺髓质内Gal-1免疫反应细胞数较对照组明显增多。此外，应激诱导的Gal-1免疫反应细胞与CD45免疫反应淋巴细胞(CD45+)位于脾和胸腺髓质内。因此，应激诱导的Gal-1立即在脾和胸腺积聚，并可能通过与生理和/或心理应激后免疫器官中的CD45+淋巴细胞结合而通过细胞凋亡来调节免疫反应。
Galectin-1 (Gal-1) is differentially expressed in normal and pathological tissues and regulates immune cell homeostasis. Restraint stress increases serum Gal-1 in rats. However, the function of stress-induced Gal-1 in serum is unknown. We determined if stress-induced Gal-1 in serum accumulates in immunocompetent organs as protection from physiological and/or psychological stress. Western blotting showed that the intensity of Gal-1 bands in stressed groups was significantly higher than that in controls. RT–PCR analysis indicated that the Gal-1 mRNA level did not increase after restraint stress. The numbers of Gal-1 immunoreactive cells in the splenic periarterial lymphatic sheath (PLS) and the thymus medulla of the stressed group were increased compared with those in controls. Furthermore, stress-induced Gal-1 immunoreactive cells corresponded to CD45 immunoreactive lymphocytes (CD45+) in the PLS of the spleen and the medulla of the thymus. Thus, stress-induced Gal-1 immediately accumulates in the spleen and thymus, and may modulate the immune response through apoptosis by binding to CD45+ lymphocytes in immune organs following physiological and/or psychological stress.