The GlxR regulon of the amino acid producer Corynebacterium glutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model
The GlxR regulon of the amino acid producer Corynebacterium glutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model
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DOI：
10.1016/j.jbiotec.2009.08.005
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发表时间：
2009-09-25
影响因子：
4.1
通讯作者：
Tauch, Andreas
中科院分区：
文献类型：
--
作者：
Kohl, Thomas A.;Tauch, Andreas
谷氨酸棒杆菌是工业上重要的氨基酸生产者，并且是棒杆菌族的新兴模式系统。将C.谷氨酸杆菌ATCC 13032编码Crp-Fnr蛋白家族的DNA结合转录因子。现有的数据表明GlxR在C.谷氨酸。我们已经使用最近发表的几个棒状杆菌的全基因组注释，从比较基因组学方法中获得GlxR介导的转录调控的进一步证据。生物信息学策略检测到22个基因属于一个建议GlxR核心调节棒杆菌。纯化的GlxR蛋白与40-mer寡核苷酸的结合，所述寡核苷酸代表来自C.谷氨酸杆菌ATCC 13032通过电泳迁移率变动测定在体外验证。基于目前的数据，一个可靠的基因组规模的预测GlxR结合位点进行，表明约14%的注释C。GlxR可能直接调控glutamicum基因的转录。GlxR介导的调控相互作用与CoryneRegNet中存储的数据的整合使得能够重建C.谷氨酸。该网络模型具有层次化和模块化的结构，其特征在于每个功能模块中存在主调节器，GlxR作为主导调节中心。(C)2009爱思唯尔有限公司版权所有。
Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C. glutamicum ATCC 13032 encodes a DNA binding transcription factor of the Crp-Fnr protein family. Available data indicated a prominent role of GlxR in the transcriptional regulatory network of C. glutamicum. We have used recently published whole-genome annotations of several corynebacteria to derive further evidence for GlxR-mediated transcriptional regulation from a comparative genomics approach. A bioinformatic strategy detected 22 genes belonging to a proposed GlxR core regulon in corynebacteria. Binding of purified GlxR protein to 40-mer oligonucleotides representing predicted GlxR binding sites in the upstream region of core genes from C. glutamicum ATCC 13032 was verified in vitro by electrophoretic mobility shift assays. Based on current data, a reliable genome-scale prediction of GlxR binding sites was performed, indicating that about 14% of the annotated C. glutamicum genes might be under direct transcriptional control by GlxR. Integration of GlxR-mediated regulatory interactions with the data stored in CoryneRegNet enabled the reconstruction of a refined transcriptional regulatory network model for C. glutamicum. This network model exhibited a hierarchical and modular structure and is characterized by the presence of master regulators in each functional module, with GlxR serving as the dominating regulatory hub. (C) 2009 Elsevier B.V. All rights reserved.