Tomato abiotic stress enhanced tolerance by trehalose biosynthesis
Tomato abiotic stress enhanced tolerance by trehalose biosynthesis
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DOI：
10.1016/j.plantsci.2005.02.026
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发表时间：
2005-07-01
期刊：
影响因子：
5.2
通讯作者：
Culiánez-Macià, FA
中科院分区：
文献类型：
--
作者：
Cortina, C;Culiánez-Macià, FA
海藻糖是葡萄糖的非还原性二糖，与对不同胁迫条件的耐受性相关。通过引入酵母海藻糖-6-磷酸合酶（TPS1）基因产生转基因番茄植物，所述酵母海藻糖-6-磷酸合酶（TPS1）基因在花椰菜花叶病毒调节序列（CaMV35S）的控制下用于在植物中表达。利用农杆菌介导的转移，该基因被整合到基因组DNA中，并在番茄品种（Lycopersicon esculentum cv. UC82B植物。转TPS1基因番茄植株表现出多效性变化，如粗枝、硬叶、直立枝和异常根的发育。此外，转基因TPS1番茄植株的叶片显示出比野生型植株更高的叶绿素和淀粉含量。在干旱、盐和氧化胁迫下，TPS 1番茄植物的耐受性比野生型有所提高，这表明海藻糖生物合成产生的碳水化合物变化与胁迫反应有关。这些结果表明，在胁迫和非胁迫条件下，通过海藻糖的生物合成来提高番茄对非生物胁迫的耐受性而不降低生产力是可行的。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Trehalose is a nonreducing disaccharide of glucose that has been correlated with tolerance to different stress conditions. Transgenic tomato plants were generated by the introduction of the yeast trehalose-6-phosphate synthase (TPS1) gene, under the control of the cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. Using Agrobacterium-mediated transfer, the gene was incorporated into the genomic DNA and constitutively expressed in Lycopersicon esculentum cv. UC82B plants. TPS1 transgenic tomato plants exhibited pleiotropic changes Such as thick shoots, rigid dark-green leaves, erected branches and an aberrant root development. Additionally, leaves of transgenic TPS1 tomato plants showed a chlorophyll and starch content higher to wild-type plants. Under drought, salt and oxidative stress TPS1 tomato plants improved tolerance than wild type, suggesting that carbohydrate alterations produced by trehalose biosynthesis be linked to the stress response. These results indicate the feasibility of engineering tomato for increased tolerance of abiotic stress, without decreasing productivity, under both stress and nonstress conditions through trehalose biosynthesis. (c) 2005 Elsevier Ireland Ltd. All rights reserved.