DNA aneuploidy and dysplasia in oral potentially malignant disorders: Association with cigarette smoking and site
DNA aneuploidy and dysplasia in oral potentially malignant disorders: Association with cigarette smoking and site
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DOI：
10.1016/j.oraloncology.2009.03.008
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发表时间：
2009-10-01
期刊：
影响因子：
4.8
通讯作者：
Gandolfo, Sergio
中科院分区：
文献类型：
--
作者：
Pentenero, Monica;Giaretti, Walter;Gandolfo, Sergio
到目前为止，仍然没有可靠的口腔潜在恶性疾病(PMD)生物标志物来预测发展为鳞状细胞癌(SCC)的风险。在一项针对PMDS患者的前瞻性临床试验中，利用皮肤病刮片获得的新鲜样本，对60例PMD患者进行了DNA含量流式细胞术(DNA FCM)检测。其中6/42例无异型增生，但有DNA异倍体，8/18例同时有异型增生和异倍体(p=0.02)。将本组病例中最常见的两个部位--舌和颊粘膜相比较，发育不良的PMD主要位于舌部(p=0.01)。与不吸烟者相比，吸烟者在口腔粘膜优先发展为PMD的年龄更小(p=0.002)，其发育异常的PMD比不吸烟者少(p=0.01)。在吸烟者中，异型增生与DNA异倍体显著相关(p=0.03)。目前的数据表明，非整倍体是口腔癌发生的早期事件，烟草的影响因部位和患者年龄的不同而不同。当通过刮除获得PMD样本的DNA FCM时，可以用一种微创、快速、廉价的方法覆盖大范围的区域。此外，这些样本的DNA FCM似乎很容易进行常规分析。DNA FCM在预测PMD转化风险中的作用有待于对大量PMD的进一步研究。(C)2009爱思唯尔有限公司。保留所有权利。
To date there are still no reliable biomarkers for oral potentially malignant disorders (PMDs) to predict the risk of progression to squamous cell carcinoma (SCC). Within a prospective clinical trial of patients with PMDs, DNA content flow cytometry (DNA FCM) was evaluated for 60 PMDs using fresh samples obtained by a dermatological curette. There were 6/42 PMDs without dysplasia, but with DNA aneuploidy, versus 8/18 with both dysplasia and aneuploidy (p = 0.02). When the tongue and the buccal mucosa, the two most common sites in the present series of cases were compared, dysplastic PMDs were mainly located on the tongue (p = 0.01). Tobacco smokers, who preferentially developed PMDs in the buccal mucosa at a younger age than non-smokers (p = 0.002), had fewer dysplastic PMDs than did nonsmokers (p = 0.01). Dysplasia was significantly linked to DNA aneuploidy (p = 0.03) in smokers. The present data suggest that aneuploidy is an early event in oral carcinogenesis and that the influence of tobacco varies according to subsite and patient age. When DNA FCM of PMD samples are obtained by curette scraping, extensive areas can be covered with a minimally invasive, rapid, inexpensive procedure. Moreover DNA FCM of these samples appears easy amenable to routine analysis. Further research on larger numbers of PMDs should be carried out to determine whether DNA FCM plays a role in the prediction of risk of PMD transformation. (C) 2009 Elsevier Ltd. All rights reserved.