Single-channel properties of the recombinant skeletal muscle Ca2+ release channel (ryanodine receptor).
Single-channel properties of the recombinant skeletal muscle Ca2+ release channel (ryanodine receptor).
复制标题
重组骨骼肌 Ca2 释放通道（兰尼碱受体）的单通道特性。
DOI：
10.1016/s0006-3495(97)78221-3
复制
发表时间：
1997
影响因子：
3.4
通讯作者：
D. Maclennan
中科院分区：
文献类型：
--
作者：
S. Chen;P. Leong;J. Imredy;C. Bartlett;L. Zhang;D. Maclennan
我们报道了编码兔骨骼肌肌浆网钙释放通道(兰尼定受体)的全长cDNA在HEK-293细胞中的瞬时表达。用单通道记录的方法研究了3-[(3-cholamidopropyl)dimethylammonio]-1-propane磺酸盐溶解和蔗糖梯度纯化的重组钙释放通道的单通道特性。以对称的250 mM KCl为传导离子时，重组钙离子释放通道的K+电导为780ps，50 mM管腔内钙离子的电导为116ps。重组通道的开放时间很短，开放时间常数约为0.22ms。重组Ca~(2+)释放通道对Ca~(2+)的通透性大于对K~+的通透性，p~(2+)/P~(2+)比值为6.8。重组Ca~(2+)释放通道对不同浓度的Ca~(2+)具有双相反应，微摩尔Ca~(2+)激活该通道，毫摩尔Ca~(2+)抑制该通道。重组通道可被ATP和咖啡因激活，被镁离子和Ru红抑制，并被兰诺定修饰。大多数重组通道是不对称阻断的，从通道的管腔到细胞质一侧单向传导电流。这些数据表明，在HEK-293细胞中表达的重组钙释放通道的性质与天然通道非常相似，如果不是相同的话。
We report transient expression of a full-length cDNA encoding the Ca2+ release channel of rabbit skeletal muscle sarcoplasmic reticulum (ryanodine receptor) in HEK-293 cells. The single-channel properties of the 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate-solubilized and sucrose gradient-purified recombinant Ca2+ release channels were investigated by using single-channel recordings in planar lipid bilayers. The recombinant Ca2+ release channel exhibited a K+ conductance of 780 pS when symmetrical 250 mM KCl was used as the conducting ion and a Ca2+ conductance of 116 pS in 50 mM luminal Ca2+. Opening events of the recombinant channels were brief, with an open time constant of approximately 0.22 ms. The recombinant Ca2+ release channel was more permeable to Ca2+ than to K+, with a pCa2+/pK+ ratio of 6.8. The response of the recombinant Ca2+ release channel to various concentrations of Ca2+ was biphasic, with the channel being activated by micromolar Ca2+ and inhibited by millimolar Ca2+. The recombinant channels were activated by ATP and caffeine, inhibited by Mg2+ and ruthenium red, and modified by ryanodine. Most recombinant channels were asymmetrically blocked, conducting current unidirectionally from the luminal to the cytoplasmic side of the channel. These data demonstrate that the properties of recombinant Ca2+ release channel expressed in HEK-293 cells are very similar, if not identical, to those of the native channel.
鸡骨骼肌兰尼碱受体亚型：离子通道特性。
影响因子：
3.4
作者：
Percival,AL;Williams,AJ;Kenyon,JL;Grinsell,MM;Airey,JA;Sutko,JL
通讯作者：
Sutko,JL
FK506 结合蛋白介导的骨骼肌兰尼碱受体的校正。
DOI：
10.1016/s0006-3495(95)80109-8
发表时间：
1995
期刊：
Biophysical journal.
影响因子：
--
作者：
Ma,J;Bhat,MB;Zhao,J
通讯作者：
Zhao,J